|
Status |
Public on May 07, 2012 |
Title |
FLASH siRNA 48 hrs flash.6.48.abh |
Sample type |
RNA |
|
|
Source name |
SW480
|
Organism |
Homo sapiens |
Characteristics |
time point: 48 hrs cell line: SW480 treatment: FLASH siRNA
|
Extracted molecule |
total RNA |
Extraction protocol |
RNeasy Mini Kit (Qiagen) + RNase-Free DNase Set (Qiagen)
|
Label |
Cy3
|
Label protocol |
Quick Amp Labeling Kit (Agilent Technologies, Cat. #: 5190-0442), including MMLV-RT and oligo dT-Promoter primers for cDNA sinthesis, and T7 RNA Polymerase, Cy3-CTP and NTPs for labeling.
|
|
|
Hybridization protocol |
Agilent Gene Expression Hybridization Kit (Agilent Technologies, Cat. #: 5188-5242). Hybridizations were performed on SureHyb chambers on a rotator oven at 65º C for 17 hours.
|
Scan protocol |
Agilent G2565BA
|
Description |
aneuploid, colon adenocarcinoma cell line
|
Data processing |
log2 transformation of signal intensity, quantile normalization
|
|
|
Submission date |
May 19, 2011 |
Last update date |
May 07, 2012 |
Contact name |
Frank Kramer |
E-mail(s) |
frank.kramer@med.uni-goettingen.de
|
Organization name |
University Medical Center Göttingen
|
Department |
Department of Medical Statistics
|
Street address |
Humboldtallee 32
|
City |
Goettingen |
ZIP/Postal code |
37073 |
Country |
Germany |
|
|
Platform ID |
GPL4133 |
Series (1) |
GSE29405 |
Systems-wide RNAi analysis of CASP8AP2 / FLASH |
|