|
| Status |
Public on Mar 01, 2006 |
| Title |
Med13 ORF-1 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
CHIP IP Med13-1 ORF
|
| Organism |
Schizosaccharomyces pombe |
| Characteristics |
IP sample med13-TAP
|
| Extracted molecule |
genomic DNA |
| Label |
cy5
|
| |
|
| Channel 2 |
| Source name |
WCE
|
| Organism |
Schizosaccharomyces pombe |
| Characteristics |
WCE of Med13-TAP
|
| Extracted molecule |
genomic DNA |
| Label |
cy3
|
| |
|
| |
| Description |
IgG beads were used for ChIP of chromatin extracts from Med13-TAP cells. Cell cultures were then grown to mid logarithmic growth phase (5 x106 cells/ml) in rich medium before fixation and ChIP. The resulting ChIP DNA was
labeled with Cy5. The wCE control ChIP DNA was labeled with Cy3 and imultaneously hybridized with the Cy5 labeled DNA to the IGR+ORF microarrays to produce genome wide mediator occupancy profile.
|
| Data processing |
cy5/cy3
|
| |
|
| Submission date |
Aug 26, 2005 |
| Last update date |
Dec 27, 2005 |
| Contact name |
Claes M Gustafsson |
| E-mail(s) |
claes.gustafsson@medkem.gu.se
|
| Organization name |
Gothenburg University
|
| Department |
Department of Medical biochemistry and cell biology
|
| Street address |
Medcinaregatan 9A
|
| City |
Gothenburg |
| ZIP/Postal code |
405 30 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL2789 |
| Series (1) |
| GSE3213 |
Genome-wide occupancy profile of mediator and the srb8-11 module reveals interactions with coding regions. |
|
