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Sample GSM721224 Query DataSets for GSM721224
Status Public on Sep 13, 2011
Title H3K79me2_mLSC_ChIPSeq
Sample type SRA
Source name murine L-GMP
Organism Mus musculus
Characteristics strain: C57BL/6
genotype: MLL-AF9
cell origin: MLL-AF9 Leukemic Stem Cell (IL-7R-Lin-Sca-1-c-Kit+CD34+FcgammaII/III+) from GMP-derived leukemia
chip antibody: H3K79me2
Treatment protocol Cells were subjected to ChIP-Seq using an antibody specific for H3K4me3, H3K27me3, H3K36me3, H3K79me2 or streptavidin beads (for biotinylated MLL-AF9 targets).
Growth protocol GMP-like leukaemic cells (L-GMP) were isolated by flow cytometry from GMP derived MLL-AF9 primary leukemias.
Extracted molecule genomic DNA
Extraction protocol ChIP DNA libraries were made following the Illumina ChIP-seq library preparation kit.
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina Genome Analyzer IIx
Description Chromatin IP against H3K79me2
Data processing Sequence reads were aligned to the mouse genome assembly mm8 or human genome assembly hg18 using Bowtie. ChIP-Seq signal was quantified as total number of reads per million in the region of interest. An empirical background distribution model of reads was constructed to find the significance level of signal in a given region.
Submission date May 06, 2011
Last update date May 15, 2019
Contact name Amit Sinha
Phone 617-582-7579
Organization name Dana-Farber Cancer Institute
Department Pediatric Oncology
Lab Armstrong Lab
Street address 44 Binney St
City Boston
State/province MA
ZIP/Postal code 02135
Country USA
Platform ID GPL11002
Series (1)
GSE29130 Epigenetic profiling of hematopoietic stem cells and leukemia stem cells
SRA SRX061985
BioSample SAMN00264825

Supplementary file Size Download File type/resource
GSM721224_L-GMP_947.H3K79me2.a_m_1_best_strata.mm8.wig.gz 12.5 Mb (ftp)(http) WIG
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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