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Sample GSM721219 Query DataSets for GSM721219
Status Public on Sep 13, 2011
Title H3K36me3_mHSC_ChIPSeq
Sample type SRA
Source name murine HSC
Organism Mus musculus
Characteristics strain: C57BL/6
genotype: wild type
age: 6-8 weeks
cell origin: murine LSK cells (IL-7R-Lin-Sca-1+c-Kit+)
chip antibody: H3K36me3
Treatment protocol Cells were subjected to ChIP-Seq using an antibody specific for H3K4me3, H3K27me3, H3K36me3, H3K79me2 or streptavidin beads (for biotinylated MLL-AF9 targets).
Growth protocol LSK cells were isolated by flow cytometry from bone marrow of wild type C57BL/6 (6-8 wks).
Extracted molecule genomic DNA
Extraction protocol ChIP DNA libraries were made following the Illumina ChIP-seq library preparation kit.
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina HiSeq 2000
Description Chromatin IP against H3K36me3
Data processing Sequence reads were aligned to the mouse genome assembly mm8 or human genome assembly hg18 using Bowtie. ChIP-Seq signal was quantified as total number of reads per million in the region of interest. An empirical background distribution model of reads was constructed to find the significance level of signal in a given region.
Submission date May 06, 2011
Last update date May 15, 2019
Contact name Amit Sinha
Phone 617-582-7579
Organization name Dana-Farber Cancer Institute
Department Pediatric Oncology
Lab Armstrong Lab
Street address 44 Binney St
City Boston
State/province MA
ZIP/Postal code 02135
Country USA
Platform ID GPL13112
Series (1)
GSE29130 Epigenetic profiling of hematopoietic stem cells and leukemia stem cells
SRA SRX061980
BioSample SAMN00264820

Supplementary file Size Download File type/resource
GSM721219_LSK.H3K36me3.a_m_1_best_strata.mm8.wig.gz 67.5 Mb (ftp)(http) WIG
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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