NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM721212 Query DataSets for GSM721212
Status Public on Sep 13, 2011
Title MLL-AF9_ChIPSeq
Sample type SRA
 
Source name MLL-AF9 transformed blasts
Organism Mus musculus
Characteristics strain: C57BL/6
cell origin: 5-FU treated BM cells transduced with HA-Bio-MLL-AF9 and BirA
chip antibody: streptavidin beads
Treatment protocol Cells were subjected to ChIP-Seq using an antibody specific for H3K4me3, H3K27me3, H3K36me3, H3K79me2 or streptavidin beads (for biotinylated MLL-AF9 targets).
Growth protocol Murine bone marrow cells were transformed with biotinylated MLL-AF9, harvested after two rounds of replating.
Extracted molecule genomic DNA
Extraction protocol ChIP DNA libraries were made following the Illumina ChIP-seq library preparation kit.
 
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina Genome Analyzer IIx
 
Description Chromatin IP using streptavidin beads against biotinylated MLL-AF9
Data processing Sequence reads were aligned to the mouse genome assembly mm8 or human genome assembly hg18 using Bowtie. ChIP-Seq signal was quantified as total number of reads per million in the region of interest. An empirical background distribution model of reads was constructed to find the significance level of signal in a given region.
 
Submission date May 06, 2011
Last update date May 15, 2019
Contact name Amit Sinha
E-mail(s) amit.sinha@childrens.harvard.edu
Phone 617-582-7579
Organization name Dana-Farber Cancer Institute
Department Pediatric Oncology
Lab Armstrong Lab
Street address 44 Binney St
City Boston
State/province MA
ZIP/Postal code 02135
Country USA
 
Platform ID GPL11002
Series (1)
GSE29130 Epigenetic profiling of hematopoietic stem cells and leukemia stem cells
Relations
SRA SRX099358
BioSample SAMN00728492

Supplementary file Size Download File type/resource
GSM721212_MLL-AF9_Bio.SDS.a_m_1_best_strata.mm8.norep_Satellite.wig.gz 35.2 Mb (ftp)(http) WIG
SRA Run SelectorHelp
Processed data provided as supplementary file
Raw data are available in SRA

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap