|
| Status |
Public on Mar 04, 2024 |
| Title |
Tgfbr2_KO_8 |
| Sample type |
SRA |
| |
|
| Source name |
lung
|
| Organism |
Mus musculus |
| Characteristics |
tissue: lung
strain: C57BL/6 CD1 mixed
genotype: HopxCreERT2+/-;Tgfbr2 fl/fl; eYFP-/-
treatment: No treatment
|
| Extracted molecule |
total RNA |
| Extraction protocol |
PureLink™ RNA Micro Kit
NEBNext®Single Cell/Low Input RNA Library Prep Kit for Illumina®
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Fastq files were align to the mm39 reference genome using STAR and default parameters
Ensmebl v104 gene annotations were used for gene counts, gene counts were obtain using STAR
Raw gene counts were transformed using 'LIMMA-Voom' method and differential gene expression conducted using LIMMA.
Assembly: mm39
Supplementary files format and content: tab-delimited text files include VOOM adjusted cpms values for each Sample
|
| |
|
| Submission date |
Apr 21, 2023 |
| Last update date |
Mar 04, 2024 |
| Contact name |
Michael Patrick Morley |
| E-mail(s) |
mmorley@pennmedicine.upenn.edu
|
| Phone |
215-898-2026
|
| Organization name |
Perelman School of Medicine at the University of Pennsylvania
|
| Department |
Penn Cardiovascular Institute
|
| Street address |
3400 Civic Center Blvd, Bldg 421
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
|
| Relations |
| BioSample |
SAMN34284963 |
| SRA |
SRX20044855 |
