|
| Status |
Public on Mar 01, 2006 |
| Title |
Med7 IGR-6 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
CHIP IP Med7-6 IGR
|
| Organism |
Schizosaccharomyces pombe |
| Characteristics |
IP sample med7-TAP
|
| Extracted molecule |
genomic DNA |
| Label |
cy5
|
| |
|
| Channel 2 |
| Source name |
WCE
|
| Organism |
Schizosaccharomyces pombe |
| Characteristics |
WCE of Med7-TAP
|
| Extracted molecule |
genomic DNA |
| Label |
cy3
|
| |
|
| |
| Description |
IgG beads were used for ChIP of chromatin
extracts from Med7-TAP cells. Cell cultures
were then grown to mid logarithmic growth
phase (5 x106 cells/ml) in rich medium before
fixation and ChIP. The resulting ChIP DNA was
labeled with Cy5. The wCE control ChIP DNA was
labeled with Cy3 and simultaneously hybridized
with the Cy5 labeled DNA to the IGR+ORF
microarrays to produce genome wide mediator
occupancy profile.
|
| Data processing |
cy5/cy3
|
| |
|
| Submission date |
Aug 26, 2005 |
| Last update date |
Dec 27, 2005 |
| Contact name |
Claes M Gustafsson |
| E-mail(s) |
claes.gustafsson@medkem.gu.se
|
| Organization name |
Gothenburg University
|
| Department |
Department of Medical biochemistry and cell biology
|
| Street address |
Medcinaregatan 9A
|
| City |
Gothenburg |
| ZIP/Postal code |
405 30 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL2789 |
| Series (1) |
| GSE3213 |
Genome-wide occupancy profile of mediator and the srb8-11 module reveals interactions with coding regions. |
|
