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Sample GSM7178956 Query DataSets for GSM7178956
Status Public on May 29, 2024
Title PEL_3d-2
Sample type SRA
Source name Hypothalamus
Organism Mus musculus
Characteristics tissue: Hypothalamus
treatment: Pituitary stalk electrical lesion (PEL)
time: Day 3
Growth protocol Brain tissue was removed and stored in liquid nitrogen
Extracted molecule total RNA
Extraction protocol RNA was harvested using Rneasy mini plus kit (Qiagen). 1.3 ug of total RNA was used for the construction of sequencing libraries.
RNA libraries for RNA-seq were prepared using SMARTER mRNA-Seq Library Prep Kit following manufacturer's protocols.
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
Data processing CLC Genomics Workbench v 11.0.1
Sequence reads were trimmed for adaptor sequence/low-quality sequence using CLC genomic benchwork (parameter- Quality limit: 0.05)
Trimmed sequence reads were mapped to GrCM38/mm10 using CLC genomic benchwork (parameters- mismath cost: 2 insertion cost: 3 deletion cost: 3 length fraction: 0.8 similarity fraction: 0.8)
Read count extraction and normalization were performed using CLC genomic benchwork
Assembly: mm10
Supplementary files format and content: tab-delimited text files include RPKM values for each Sample
Submission date Apr 16, 2023
Last update date May 29, 2024
Contact name guangsen wu
Phone 15521009401
Organization name Southern Medical University Nanfang Hospital
Street address 广东省广州市广州大道北1838号南方医科大学南方医院
City guangzhou
ZIP/Postal code 051000
Country China
Platform ID GPL24247
Series (1)
GSE229838 Effect of pituitary stalk electrical lesion (PEL) and growth hormone (GH) treatment on hypothalamic and hippocampal gene expression
BioSample SAMN34209500
SRA SRX19983808

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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