|
| Status |
Public on May 17, 2024 |
| Title |
JHU083, CD45- tumor cells, mouse 725 |
| Sample type |
SRA |
| |
|
| Source name |
Sub-cutaneous tumor
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Sub-cutaneous tumor
cell line: B6CaP
cell type: CD45- sorted cells
genotype: Male C57BL/6J
treatment: JHU083 oral gavage
|
| Treatment protocol |
Each mouse was treated with either PBS (control) or JHU083 by oral gavage.
|
| Growth protocol |
B6CaP cells were injected subcutaneously into the flank region of each mouse.
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
RNA was extracted from sorted cells (Live CD45+ or CD45-) by first using the NextGEM protocol on 10X Chromium X, following manufacturer's instructions.
Library construction was performed according to manufacturer's instructions (single cell 3' v3.1 protocol, 10X Genomics)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic single cell |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
10X Genomics
|
| Data processing |
Demultiplexing, barcode processing, and gene counting and aggregation were made using the Cell Ranger software v7.0.0
Assembly: mm10
Supplementary files format and content: HDF5 files from the cellranger count pipeline, each containing the count data for each sample
|
| |
|
| Submission date |
Apr 07, 2023 |
| Last update date |
May 17, 2024 |
| Contact name |
Alex Jea-Eun Lee |
| E-mail(s) |
alee262@jhmi.edu
|
| Organization name |
Johns Hopkins University
|
| Street address |
1650 Orleans Street
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21231 |
| Country |
USA |
| |
|
| Platform ID |
GPL24247 |
| Series (2) |
| GSE229223 |
Glutamine antagonist JHU083 reprograms immunosuppressive tumor-associated macrophages to drive tumor immunity in urologic cancers, single-cell RNA-seq |
| GSE230162 |
Glutamine antagonist JHU083 reprograms immunosuppressive tumor-associated macrophages to drive tumor immunity in urologic cancer |
|
| Relations |
| SRA |
SRX19907909 |
| BioSample |
SAMN34116524 |
