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Sample GSM7156940 Query DataSets for GSM7156940
Status Public on Apr 01, 2024
Title KW148-gb102c1
Sample type SRA
 
Source name blood
Organism Homo sapiens
Characteristics tissue: blood
cell type: CD34+ cells
genotype: WT
treatment: HBG2i-WT
Extracted molecule genomic DNA
Extraction protocol high molecular weight genomic DNA was extracted, and 5' and 3' phosphates were removed. The b-globin gene cluster was enriched by Cas9-RNPs digestion of two 10 kb fragments. PacBio SMRTbells were ligated to fragments, pooled and sequenced on a PacBio Sequel IIe
PacBio libraries were generated using the No Amp protocol
 
Library strategy OTHER
Library source genomic
Library selection other
Instrument model Sequel II
 
Description bc1002--bc1002.bam
PacBio No-Amp Targeted sequencing
PacBio_Results.csv
Data processing Instrument model: PacBio Sequel IIe
FASTQ files were used as input files to align to chromosome 11 (hg38) by minimap2 (v.2.24)
Assembly: hg38
Supplementary files format and content: csv file with variant calling
 
Submission date Apr 07, 2023
Last update date Apr 01, 2024
Contact name Kristen Seim
E-mail(s) kristen.seim@gmail.com
Organization name GraphiteBio
Street address 201 Haskins Way
City San Francisco
State/province CA
ZIP/Postal code 94080
Country USA
 
Platform ID GPL28352
Series (1)
GSE229212 Development of a ß-Globin Gene Replacement Strategy as a Therapeutic Approach for ß-Hemoglobinopathies
Relations
BioSample SAMN34115875
SRA SRX19907174

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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