|
| Status |
Public on Apr 01, 2024 |
| Title |
gb102 Engraftment study; Donor 328 RNP only |
| Sample type |
SRA |
| |
|
| Source name |
blood
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: blood
cell type: CD34+ cells
genotype: WT
treatment: RNP
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
high molecular weight genomic DNA was extracted, and 5' and 3' phosphates were removed. The b-globin gene cluster was enriched by Cas9-RNPs digestion of two 10 kb fragments. PacBio SMRTbells were ligated to fragments, pooled and sequenced on a PacBio Sequel IIe
PacBio libraries were generated using the No Amp protocol
|
| |
|
| Library strategy |
OTHER |
| Library source |
genomic |
| Library selection |
other |
| Instrument model |
Sequel II |
| |
|
| Description |
328_RNP
PacBio No-Amp Targeted sequencing
PacBio_Results.csv
|
| Data processing |
Instrument model: PacBio Sequel IIe
FASTQ files were used as input files to align to chromosome 11 (hg38) by minimap2 (v.2.24)
Assembly: hg38
Supplementary files format and content: csv file with variant calling
|
| |
|
| Submission date |
Apr 07, 2023 |
| Last update date |
Apr 01, 2024 |
| Contact name |
Kristen Seim |
| E-mail(s) |
kristen.seim@gmail.com
|
| Organization name |
GraphiteBio
|
| Street address |
201 Haskins Way
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94080 |
| Country |
USA |
| |
|
| Platform ID |
GPL28352 |
| Series (1) |
| GSE229212 |
Development of a ß-Globin Gene Replacement Strategy as a Therapeutic Approach for ß-Hemoglobinopathies |
|
| Relations |
| BioSample |
SAMN34115878 |
| SRA |
SRX19907171 |
