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Sample GSM7156898 Query DataSets for GSM7156898
Status Public on May 21, 2024
Title Steady state wild type bone marrow myeloid progenitors
Sample type SRA
Source name Bone Marrow
Organism Mus musculus
Characteristics tissue: Bone Marrow
strain: C57BL6
cell type: Myeloid progenitors
genotype: wild type
treatment: none
Extracted molecule total RNA
Extraction protocol Myeloid progenitors (lineage-negative c-kit+ IL7Ra-) were sorted using a BD FACSAria III (BD Biosciences) cell sorter as described in Rosu et al.(DOI: 10.1084/jem.20200662 ) and were resuspended in Ca and Mg free PBS containing 0.4mg/ml of UltraPure™ BSA (Thermofisher, AM2616).In total, 16,000 sorted cells were submitted to the 10X Genomics pipeline for encapsulation aiming for a recovery of 10,000 sequenced single cells as described in the manufacturer’s instructions.
Sequencing libraries were prepared using Chromium Single Cell 3' Reagent Kit V3 (10X Genomics) as per manufacturer’s instructions and cDNA quality and quantity were controlled using Agilent High Sensitivity DNA Kit (Agilent) on a 2100 Bioanalyzer (Agilent).
Library strategy RNA-Seq
Library source transcriptomic single cell
Library selection cDNA
Instrument model Illumina NovaSeq 6000
Description 10x Genomics
Data processing Demultiplexing, barcoded processing, gene counting and aggregation were made using the Cell Ranger software v3.0.2 (
Assembly: mm10
Supplementary files format and content: Tab-separated values files and matrix files
Submission date Apr 07, 2023
Last update date May 21, 2024
Contact name Christophe J Desmet
Organization name University of Liege
Lab Cellular and Molecular Immunology
Street address B34, Avenue de l'Hopital 1
City Liege
ZIP/Postal code 4000
Country Belgium
Platform ID GPL24247
Series (2)
GSE229208 Gene expression profile at single cell level of Bone Marrow Myeloid Progenitors
GSE249011 Bulk and single-cell RNA sequencing of human and murine eosinophil lineage cells
SRA SRX19907286
BioSample SAMN34115960

Supplementary file Size Download File type/resource
GSM7156898_barcodes.tsv.gz 20.0 Mb (ftp)(http) TSV
GSM7156898_features.tsv.gz 191.1 Kb (ftp)(http) TSV
GSM7156898_matrix.mtx.gz 200.5 Mb (ftp)(http) MTX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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