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Status |
Public on Aug 08, 2023 |
Title |
Mouse Lung tumor_KrasLA1;DicerS2D_rep1 |
Sample type |
RNA |
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Source name |
primary lung tumor
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Organism |
Mus musculus |
Characteristics |
gender: male age in weeks: 37w strain: C57B6J tissue: lung tumor genotype: KrasLA1/+;Dicer1S2D/+
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Treatment protocol |
No treatment. This are genetically modified mouse models that carry oncogenic Kras G12D (Kras LA1/+); and phosphomimetic Dicer1 wherein serines at positions 1712 and 1836 were replaced to aspartic acid in a oncogenic Kras G12D background (KrasLA1/+;Dicer1S2D/+)
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Growth protocol |
30 to 37 weeks old mice from both genotypes were euthanized following IACUC protocol
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Extracted molecule |
total RNA |
Extraction protocol |
Lungs were removed from mouse. Then tumors were identified and collected under the dissecting microscope. 50ug of tumors were homogenized in 1 mL of Trizol (Ambion, #15596018). Total RNA was purified following miRNeasy Mini Kit (Qiagen, #217004). 100ng of total RNA was used for nCounter Nanostring miRNA (NS_M_MIR_V1.5) assay.
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Label |
miRNA reported and capture probes
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Label protocol |
nCounter Nanostring mouse miRNA (NS_M_MIR_V1.5) assay with capture probes which are complementary to miRNAs sequence
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Hybridization protocol |
Hybridization was performed following nCounter Nanostring miRNA (NS_M_MIR_V1.5) protocol in where miRNA reporter codeset, hybridization buffer and miRNA capture probe set was added to each sample.
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Scan protocol |
Nanostring nCounter instrunent
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Description |
miRNAs were assayed using Ncounter Nanostring mouse miRNAs v1.5
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Data processing |
Results were analyzed in nCounter Analysis Software v4.0. Statistical analysis was performed using two-tailed t-test and Benjamin-Yekutieli for false discovery rate.
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Submission date |
Apr 04, 2023 |
Last update date |
Aug 08, 2023 |
Contact name |
Raisa Reyes-Castro |
E-mail(s) |
RAReyes2@mdanderson.org
|
Organization name |
UT Health MD Anderson Cancer Center
|
Department |
Genetics
|
Lab |
Swathi Arur
|
Street address |
6767 Bertner Ave
|
City |
Houston |
State/province |
Texas |
ZIP/Postal code |
77030 |
Country |
USA |
|
|
Platform ID |
GPL33314 |
Series (2) |
GSE228966 |
Phosphorylated nuclear DICER1 promotes open chromatin state and gastric cell fate in lung adenocarcinomas [Nanostring] |
GSE228968 |
Phosphorylated nuclear DICER1 promotes open chromatin state and gastric cell fate in lung adenocarcinomas |
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