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Sample GSM7108011 Query DataSets for GSM7108011
Status Public on Nov 28, 2023
Title CMP (group B) Hoxa1mut
Sample type RNA
 
Source name CMP_Hoxa1mut_r2
Organism Mus musculus
Characteristics biological replicate: 2
cell sort information: FACS Sorted BM LKS-CD34+CD16/32-
pool: B
tissue: Bone Marrow
tissue: CMP
overexpression??: Hoxa1mut
genetic background: C57BL/6
Growth protocol Cell were FACS sorted from primiary receipent mice 16 weeks post transplantation of retrovirally overexpressed immature bone marrow cells.
Extracted molecule total RNA
Extraction protocol Performed by Ramaciotti Centre for Gene Function Analysis at the University of New South Wales, Sydney, Australia in accordance with the manufacturer’s instructions.
Label biotin
Label protocol Performed by Ramaciotti Centre for Gene Function Analysis at the University of New South Wales, Sydney, Australia in accordance with the manufacturer’s instructions.
 
Hybridization protocol Performed by Ramaciotti Centre for Gene Function Analysis at the University of New South Wales, Sydney, Australia in accordance with the manufacturer’s instructions.
Scan protocol Performed by Ramaciotti Centre for Gene Function Analysis at the University of New South Wales, Sydney, Australia in accordance with the manufacturer’s instructions.
Description Common Myeloid Progenitor Hoxa1 mutant rep 2
Data processing samples were RMA normalised in GenePattern using NormalizeAffymetrixST 2.2 (RMA, core probesets, controls included,Affymetrix_MoGene_1_1_st_v1.na33.2.mm9.transcript.chip).
 
Submission date Mar 20, 2023
Last update date Nov 28, 2023
Contact name Alistair Morgan Chalk
E-mail(s) achalk@svi.edu.au, alistair.chalk@gmail.com
Organization name St Vincent's Institute of Medical Research
Department Stem Cell Regulation Unit
Lab Walkley
Street address 9 Princes st, Fitzroy
City Melbourne
State/province VIC
ZIP/Postal code 3065
Country Australia
 
Platform ID GPL11533
Series (1)
GSE227775 Modeling myelodysplastic syndromes in mice by altered Hoxa1 spliceform expression (CMP)

Data table header descriptions
ID_REF
VALUE log2 RMA normalised signal using GenePattern module NormalizeAffymetrixST.

Data table
ID_REF VALUE
10338001 11.5372
10338002 3.6399
10338003 10.1581
10338004 9.7695
10338005 1.8435
10338006 1.9854
10338007 2.2445
10338008 2.3701
10338009 5.6712
10338010 1.8154
10338011 3.2645
10338012 1.8539
10338013 1.6673
10338014 1.708
10338015 1.6831
10338016 4.4109
10338017 12.4288
10338018 3.878
10338019 2.9114
10338020 5.7286

Total number of rows: 35556

Table truncated, full table size 552 Kbytes.




Supplementary file Size Download File type/resource
GSM7108011_CMP_Hoxa1mut_r2.CEL.gz 3.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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