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Sample GSM7107981 Query DataSets for GSM7107981
Status Public on May 03, 2023
Title PAO1 ∆phrS pPhrS replicate B
Sample type SRA
 
Source name ∆phrS strain with vector for pulsed expression of PhrS-WT allele (pTK60)
Organism Pseudomonas aeruginosa
Characteristics strain: PAO1
genotype: {delta}phrS pTK60
treatment: Growth: OD ≈ 2.0 in LB + gentamicin (30 µg/mL)
treatment: 20 minutes pulse of 0.2% (w/v) arabinose
Treatment protocol Cells were harvested by centrifugation, washed with 1X PBS, and cell pellets snap frozen in a dry ice - ethanol bath.
Growth protocol Bacterial strains were grown at 37˚C , shaking at 200 rpm, in LB rich medium and samples 1&2 were collected at OD ≈ 0.5, samples 3&4 were collected at OD ≈ 2.0.
Extracted molecule total RNA
Extraction protocol Standard TRI Reagent protocol was used for RNA extraction
Libary construction was carried out based on the RNAtag-Seq methodology (Shishkin, et al., 2015) for samples 5 - 8.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model NextSeq 2000
 
Description Sample 2B
Data processing fastq records trimming (cutadapt 2.10) (Samples 5 - 8)
Quality control and adapter trimming (bcl2fastq 2.20.0.445) (Samples 9-10)
short reads mapping (bowtie2 2.4.4) (Samples 5-10)
BED file conversion (bedtools 2.30.0) (Samples 5-10)
bigwig file conversion (bedGraphToBigWig 2.9) (Samples 5-10)
Read quantification (htseq-count 2.0.2) (Samples 9-10)
Assembly: P. aerugionsa PAO1, NC_002516.2
Supplementary files format and content: Supplementary_files_format_and_content: bigwig format for samples 5 - 10.
 
Submission date Mar 20, 2023
Last update date Jun 13, 2023
Contact name Michael J Gebhardt
Organization name University of Iowa
Department Department of Microbiology and Microbiology
Lab Gebhardt
Street address 431 Newton Road, 200A EMRB
City Iowa City
State/province IA
ZIP/Postal code 52242
Country USA
 
Platform ID GPL33271
Series (2)
GSE216135 PAO1 Hfq-V RIL-seq and RNA-seq
GSE227772 PhrS pulse expression RNA-seq
Relations
BioSample SAMN33832086
SRA SRX19732831

Supplementary file Size Download File type/resource
GSM7107981_pTK60_20_B_minus.bw 8.7 Mb (ftp)(http) BW
GSM7107981_pTK60_20_B_plus.bw 7.9 Mb (ftp)(http) BW
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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