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Sample GSM7097906 Query DataSets for GSM7097906
Status Public on Nov 10, 2023
Title pdKO, dLN, rep2_rna
Sample type SRA
 
Source name Lung draining lymph nodes
Organism Mus musculus
Characteristics tissue: Lung draining lymph nodes
replicate: Rep 2
flow markers: CD44+ CD4+ CD19- CD8- Rosa26-YFP+
cell type: Activated CD4+ T cells
genotype: Zfp148 fl/fl; Zfp281 fl/fl; Cd2-Cre+
Extracted molecule total RNA
Extraction protocol Nuclei were isolated following the recommended protocol from 10X Genomics, using a 3 minute lysis time.
Chromium NextGem Single Cell ATAC + Gene Expression
 
Library strategy RNA-Seq
Library source transcriptomic single cell
Library selection cDNA
Instrument model NextSeq 2000
 
Data processing The standard 10x Genomics cellranger ARC (version 2.0.1) pipeline was used to perform data processing.
Sequenced reads were aligned to the 10x Genomics provided mouse reference sequence
Assembly: mm10
Supplementary files format and content: h5; Feature and barcode matrix
Supplementary files format and content: tsv; fragment file for ATAC fragments
Supplementary files format and content: csv; statistics
 
Submission date Mar 14, 2023
Last update date Nov 10, 2023
Contact name Remy Bosselut
E-mail(s) bosselur@helix.nih.gov
Phone 240-760-6866
Organization name National Cancer Institute, National Institute of Health
Department Laboratory of Immune Cell Biology
Lab Bosselut
Street address 9000 Rockville Pike, Building 37
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platform ID GPL30172
Series (2)
GSE207572 Zfp281 and Zfp148 control CD4+ T cell thymic development and Th2 functions
GSE227330 The transcription factor Zfp281 serves redundantly with Zfp148 to support CD4+ T cell development and functions [Papain multiome]
Relations
BioSample SAMN33761949
SRA SRX19678959

Supplementary file Size Download File type/resource
GSM7097906_pdKO_rep2_filtered_feature_bc_matrix.h5 166.9 Mb (ftp)(http) H5
GSM7097906_pdKO_rep2_raw_feature_bc_matrix.h5 189.0 Mb (ftp)(http) H5
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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