|
Status |
Public on Mar 17, 2023 |
Title |
FL318_24h_Prednisolone_Replicate 1 |
Sample type |
SRA |
|
|
Source name |
FL318
|
Organism |
Homo sapiens |
Characteristics |
cell line: FL318 cell type: human DLBCL cell line genotype: Cas9 engineered treatment: 10uM_Predisolone_24h
|
Treatment protocol |
All cells were grown at 0.5 x 10^6 cells/ml and either treated in duplicates with vehicle (DMSO), prednisolone (25nm or 10µM) for 0h 6h and 24h.
|
Growth protocol |
All cells were grown in advanced RPMI supplemented with 5% FBS with pen/strep and glutamine.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted using the Qiagen RNeasy Mini kit. Libraries were generated using TruSeq stranded mRNA library prep (Illumina).
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
NextSeq 2000 |
|
|
Description |
fl318_24h_r1 total mRNA 2x50 cycle paired-end RNA-seq
|
Data processing |
Illumina Casava1.7 software used for basecalling. Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to hg19 human genome using STAR2. Raw read counts per gene were called with Htseq-Count. FPKM normalization and median shift. Assembly: hg19 Supplementary files format and content: DLBCL_Prednisolone_RNAseq_FPKM_Normalized_Log2.txt
|
|
|
Submission date |
Mar 07, 2023 |
Last update date |
Mar 17, 2023 |
Contact name |
David Huang |
Organization name |
National Cancer Institute
|
Street address |
9000 Rockville Pike
|
City |
Bethesda |
State/province |
Maryland |
ZIP/Postal code |
20892-0001 |
Country |
USA |
|
|
Platform ID |
GPL30173 |
Series (2) |
GSE225858 |
Targeting oncogenic BCR signaling therapeutically by glucocorticoids and CSK inhibition |
GSE226862 |
RNA sequencing/Gene expression studies of prednisolone in aggressive B-cell lymphomas [JC1] |
|
Relations |
BioSample |
SAMN33619540 |
SRA |
SRX19580465 |