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Sample GSM7074115 Query DataSets for GSM7074115
Status Public on Nov 01, 2023
Title TGFBneg_Celastrolneg
Sample type SRA
 
Source name Control 6h
Organism Homo sapiens
Characteristics tissue: Skin
cell line: CCD-1077Sk
cell type: Fibroblasts
condition: Normal Skin
treatment: BSA+Citric Acid+DMSO
Treatment protocol Cells were treated with either DMSO or TGFB1 with or without Celastrol for 6h
Growth protocol CCD-1077Sk cells (>P8) was cultured in high glucose DMEM+10%FBS+1%anti-anti until 70% confluency before treatment
Extracted molecule total RNA
Extraction protocol The RNA was extracted using the TriZol extraction method.
Total RNA samples were processed using the VAHTS Total RNA-seq (H/M/R) Library Prep Kit for Illumina (PN NR603) from Vazyme Biotech Co. Ltd.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NextSeq 500
 
Data processing Partek Flow v10.0.22
fastq files were aligned to hg38 using Star 2.7.3a and annotated with Ensembl Transcripts release 100
Features were excluded if maximum reads <=11.0
Reads were normalized using SESeq2 (median ratio)
Fold changes and p-values were caluclated using DESeq2.
Assembly: hg38
Supplementary files format and content: tab-delimited text file including fold change and statistical significance value for each gene
 
Submission date Mar 01, 2023
Last update date Nov 01, 2023
Contact name Andrew Leask
E-mail(s) anl312@mail.usask.ca
Organization name University of Saskatchewan
Department College of Dentistry
Lab Leask Lab
Street address 105 Wiggins
City Saskatoon
State/province Saskatchewan
ZIP/Postal code S7N5E4
Country Canada
 
Platform ID GPL18573
Series (2)
GSE226374 The Triptryium wilfordii derivative celastrol has anti-fibrotic effects in systemic sclerosis [in vitro bulk RNA-seq]
GSE226376 The Triptryium wilfordii derivative celastrol has anti-fibrotic effects in systemic sclerosis
Relations
BioSample SAMN33553500
SRA SRX19537701

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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