|
Status |
Public on Nov 01, 2023 |
Title |
TGFBplus_Celastrol_500nM 2 |
Sample type |
SRA |
|
|
Source name |
TGFB_Celastrol 2 6h
|
Organism |
Homo sapiens |
Characteristics |
tissue: Skin cell line: CCD-1077Sk cell type: Fibroblasts condition: Normal Skin treatment: TGFB+Celastrol
|
Treatment protocol |
Cells were treated with either DMSO or TGFB1 with or without Celastrol for 6h
|
Growth protocol |
CCD-1077Sk cells (>P8) was cultured in high glucose DMEM+10%FBS+1%anti-anti until 70% confluency before treatment
|
Extracted molecule |
total RNA |
Extraction protocol |
The RNA was extracted using the TriZol extraction method. Total RNA samples were processed using the VAHTS Total RNA-seq (H/M/R) Library Prep Kit for Illumina (PN NR603) from Vazyme Biotech Co. Ltd.
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NextSeq 500 |
|
|
Data processing |
Partek Flow v10.0.22 fastq files were aligned to hg38 using Star 2.7.3a and annotated with Ensembl Transcripts release 100 Features were excluded if maximum reads <=11.0 Reads were normalized using SESeq2 (median ratio) Fold changes and p-values were caluclated using DESeq2. Assembly: hg38 Supplementary files format and content: tab-delimited text file including fold change and statistical significance value for each gene
|
|
|
Submission date |
Mar 01, 2023 |
Last update date |
Nov 01, 2023 |
Contact name |
Andrew Leask |
E-mail(s) |
anl312@mail.usask.ca
|
Organization name |
University of Saskatchewan
|
Department |
College of Dentistry
|
Lab |
Leask Lab
|
Street address |
105 Wiggins
|
City |
Saskatoon |
State/province |
Saskatchewan |
ZIP/Postal code |
S7N5E4 |
Country |
Canada |
|
|
Platform ID |
GPL18573 |
Series (2) |
GSE226374 |
The Triptryium wilfordii derivative celastrol has anti-fibrotic effects in systemic sclerosis [in vitro bulk RNA-seq] |
GSE226376 |
The Triptryium wilfordii derivative celastrol has anti-fibrotic effects in systemic sclerosis |
|
Relations |
BioSample |
SAMN33553501 |
SRA |
SRX19537700 |