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Sample GSM7055839 Query DataSets for GSM7055839
Status Public on Apr 14, 2023
Title TTX_1
Sample type SRA
 
Source name hiPSCs-derived neurons/primary culture of rat astrocytes
Organisms Homo sapiens; Rattus norvegicus
Characteristics tissue: hiPSCs-derived neurons/primary culture of rat astrocytes
cell line: iPSCs
cell type: neuron+astrocyte
genotype: WT
treatment: TTX
time: Days in vitro 49
Treatment protocol DIV 49 co-cultures were treated with 1 uM TTX for 48 hours
Growth protocol Co-cultures of iPSC-derived neurons and rat astrocytes were cultured in neurobasal medium supplemented with B-27, GlutaMAX, Primocin, NT-3, BDNF and doxycycline in humidified atmosphere with 5% CO2 at 37°C until DIV 49
Extracted molecule total RNA
Extraction protocol Quick-RNA Microprep kit (Zymo Research, R1051) according to manufacturer’s instructions.
The RNASeq libraries are prepared with KAPA Stranded mRNA-Seq Illumina® Platforms Kit (Roche) starting from 500 ng of total RNA
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
 
Description Multiple_samples_count_matrix_human
Multiple_samples_count_matrix_rat
Data processing Mapping : STAR/2.7.8a
Quantification : RSEM/1.3.0
Genome : GRCh38+Rnor6.0
Annotation : gencode39+ensembl104
 
Submission date Feb 21, 2023
Last update date Apr 14, 2023
Contact name Sofia Puvogel
E-mail(s) sofia.puvogel@gmail.com
Phone +31634357455
Organization name Radboud
Street address 1431 Heeskesacker
City Nijmegen
ZIP/Postal code 6546JK
Country Netherlands
 
Platform ID GPL27452
Series (1)
GSE225761 A model for studying homeostatic plasticity in human stem cell derived neuronal networks
Relations
BioSample SAMN33401322
SRA SRX19463404

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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