|
| Status |
Public on Jun 02, 2023 |
| Title |
Ctl_sample_B [ctl-B] |
| Sample type |
SRA |
| |
|
| Source name |
tumor tissue
|
| Organism |
Mus musculus |
| Characteristics |
tissue: tumor tissue
treatment: control antibody
sample type: frozen sample
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Tumor were collected, snap frozen and cryo-grinded. RNAs were extracted with standard kit (Macherey Nagel)
RNA-Seq library was produced from 0.17µg (170ng) RNA with Illumina TruSeq® Stranded Total RNA Library Prep Human/Mouse/Rat according to the manufacturer and then sequenced with Illumina NovaSeq6000.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
PTEN_Raw_count.csv
|
| Data processing |
Fastq files were processed with Star, v2.5.2b (mapped on the reference genome:Mus_musculus.GRCm38 (GENECODE release 25)) and read count were analysed with R (ver 4.2.0) package DeSEQ2 (ver 1.36.0).
Assembly: Mus_musculus.GRCm38 (GENECODE release 25)
Supplementary files format and content: tab delimited file containing raw count
|
| |
|
| Submission date |
Feb 21, 2023 |
| Last update date |
Jun 02, 2023 |
| Contact name |
Nicolas Rama |
| E-mail(s) |
nicolas.rama@lyon.unicancer.fr
|
| Organization name |
CRCL
|
| Street address |
28 rue Laennec
|
| City |
LYON |
| ZIP/Postal code |
69890 |
| Country |
France |
| |
|
| Platform ID |
GPL24247 |
| Series (2) |
| GSE225688 |
Netrin-1 blockade induces tumor growth inhibition and EMT reversion in endometrial cancer [Mouse_Pten] |
| GSE225691 |
Netrin-1 blockade induces tumor growth inhibition and EMT reversion in endometrial cancer. |
|
| Relations |
| BioSample |
SAMN33391637 |
| SRA |
SRX19452289 |
