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Sample GSM7054120 Query DataSets for GSM7054120
Status Public on Feb 23, 2023
Title spinal tuberculosis patient 4
Sample type RNA
 
Source name Plasma
Organism Homo sapiens
Characteristics diagnosis: spinal tuberculosis
cell type: Plasma
molecule subtype: microRNA
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from 200 μl of plasma using a miRcute miRNA extraction kit (TIANGEN, China) according to the manufacturer’s protocol.
Label Hy3™
Label protocol After quality control, the miRCURY™ Hy3™/Hy5™ Power labeling kit (Exiqon, Vedbaek, Denmark) was used according to the manufacturer’s protocol.
 
Hybridization protocol The Hy3™-labeled samples were hybridized on the miRCURYTM LNA Array (v.19.0) (Exiqon) according to the array manual.
Scan protocol using the Axon GenePix 4000B microarray scanner (Axon Instruments, Foster City, CA).
Description XT11
After quality control, the miRCURY™ Hy3™/Hy5™ Power labeling kit (Exiqon, Vedbaek, Denmark) was used according to the manufacturer’s protocol. The Hy3™-labeled samples were hybridized on the miRCURYTM LNA Array (v.19.0) (Exiqon) according to the array manual. And the data were collected using the Axon GenePix 4000B microarray scanner (Axon Instruments, Foster City, CA).
Data processing Scanned images were then imported into GenePix Pro 6.0 software (Axon) for grid alignment and data extraction. Replicated miRNAs were averaged and miRNAs that intensities>=30 in all samples were chosen for calculating normalization factor
 
Submission date Feb 20, 2023
Last update date Feb 24, 2023
Contact name Zili Wang
E-mail(s) zili.wang@nyfy.com.cn
Organization name Xi’an International Medical Center Hospital Affiliated to Northwest University
Department Spine Surgery
Street address NO.737, Xitai Road, High-tech Zone
City Xi’an
ZIP/Postal code 710000
Country China
 
Platform ID GPL33147
Series (1)
GSE225679 microRNAs expression Profiles in plasma from spinal tuberculosis patients and healthy volunteers

Data table header descriptions
ID_REF
VALUE Expressed data were corrected with the background signal and then normalized using the quantile normalization

Data table
ID_REF VALUE
4610 35.625
5250 16.55
6880 232.875
9938 522.575
10138 28.3
10306 601.95
10919 251.9
10923 158.625
10925 6328.35
10928 191.4
10936 202
10937 1.175
10943 130.925
10946 89.8
10952 355.275
10954 468.9
10955 27.825
10964 3102.7
10967 84.525
10972 619.15

Total number of rows: 1462

Table truncated, full table size 19 Kbytes.




Supplementary file Size Download File type/resource
GSM7054120_XT11.gpr.gz 947.3 Kb (ftp)(http) GPR
Processed data included within Sample table

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