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Sample GSM7032529 Query DataSets for GSM7032529
Status Public on Sep 21, 2023
Title lean_beta, biological replicate 3 (RNA-seq)
Sample type SRA
Source name FACS-sorted beta cells
Organism Mus musculus
Characteristics cell type: beta
Sex: female
status: lean
Growth protocol Pancreatic islets were isolated by injection of collagenase up the common bile duct after clamping of the Ampulla of Vater. Isolates were purified by density gradient centrifugation and dissociated by gentle trituration in 0.25% Trypsin -EDTA. mIns1-H2b-mCherry positive beta cells were collected and processed immediately for ATAC-Seq library preparation.
Extracted molecule total RNA
Extraction protocol Cells were collected in Trizol post FACS purification and isolated by a chloroform extraction, assisted by phase lock tubes. RNA was precipitated by isopropanol and cleaned up over an RNEasy microcolumn per the Qiagen's taking great care to avoid carry over of ethanol following the column washes. Elution volume was 30 microliter.
Indexed sequencing libraries were constructed using the TruSeq RNA sample prep Kit v2 per Illumina instructions.
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
Description bulk RNA expression
Data processing FastQC
STAR Aligner
Assembly: mm10
Supplementary files format and content: Raw Expression Counts
Submission date Feb 08, 2023
Last update date Sep 21, 2023
Contact name Mark O. Huising
Organization name University of California, Davis
Department Neurobiology, Physiology and Behavior
Lab Huising lab
Street address One Shields Avenue
City Davis
State/province CA
ZIP/Postal code 95616
Country USA
Platform ID GPL13112
Series (2)
GSE224797 Genome wide assessment of gene expression in alpha and beta cells from lean and obese mice
GSE224798 Genome wide assessment alpha and beta cells from lean and obese mice
BioSample SAMN33209389
SRA SRX19312484

Supplementary file Size Download File type/resource
GSM7032529_4664_chy_Pos_S27_L003.featureCounts.txt.gz 2.5 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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