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Sample GSM7032048 Query DataSets for GSM7032048
Status Public on Feb 19, 2024
Title scRNA_C
Sample type SRA
 
Source name spleen
Organism Mus musculus
Characteristics tissue: spleen
cell type: B220+IgD-
genotype: C_het_KO
Treatment protocol Mice (WT, C, K, and CK) were immunized with SRBC for 10 days before sorting splenic B220+IgD- cells.
Extracted molecule polyA RNA
Extraction protocol B220+IgD- cells are sorted from spleens of SRBC-immunized mice by FACS.
Single-cell RNA-seq libraries were prepared using the Chromium Single Cell 3' Reagent Kit according to 10x Genomics specifications.
 
Library strategy RNA-Seq
Library source transcriptomic single cell
Library selection cDNA
Instrument model Illumina NovaSeq 6000
 
Data processing Cellranger 3.0
Seurat 4.0
Slingshot 2.4.0
Assembly: mm10
Supplementary files format and content: cellranger 3.0 barcode / features / matrix files
Supplementary files format and content: Seurat object with all cells and celltype metadata
Supplementary files format and content: Table of cells used for pseudotime with pseudotime values and deciles
 
Submission date Feb 07, 2023
Last update date Feb 19, 2024
Contact name Christopher Russell Chin
E-mail(s) chc2077@med.cornell.edu
Phone 3393640514
Organization name Weill Cornell
Lab Melnick Lab
Street address 413 E 69th Street, Belfer Building, BB-1462
City New York City
State/province NY
ZIP/Postal code 10021
Country USA
 
Platform ID GPL24247
Series (2)
GSE224513 Cooperative super-enhancer inactivation caused by heterozygous loss of CREBBP and KMT2D skews B-cell fate decisions and accelerates development of T-cell depleted lymphomas.
GSE224782 Cooperative super-enhancer inactivation caused by heterozygous loss of CREBBP and KMT2D skews B-cell fate decisions and accelerates development of T-cell depleted lymphomas (scRNA-Seq)
Relations
BioSample SAMN33193486
SRA SRX19305847

Supplementary file Size Download File type/resource
GSM7032048_CK_D10_CHET_barcodes.tsv.gz 49.5 Kb (ftp)(http) TSV
GSM7032048_CK_D10_CHET_features.tsv.gz 272.8 Kb (ftp)(http) TSV
GSM7032048_CK_D10_CHET_matrix.mtx.gz 46.1 Mb (ftp)(http) MTX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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