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Sample GSM7024828 Query DataSets for GSM7024828
Status Public on Feb 19, 2024
Title GCB_BK_rep4
Sample type SRA
 
Source name spleen
Organism Mus musculus
Characteristics tissue: spleen
cell type: GCB cell
genotype: BCL+K_het_KO
Treatment protocol Mice (WT, C, K, and CK) were immunized with SRBC for 10 days before collecting CBs and CCs. Mice (BCL2, BCL2+C, BCL2+K, BCL+CK) were immunized with SRBC once every 3 weeks (starting at 56 days post-BMT) to induce continuous GC formation and lymphomagenesis, GC B cells were sorted at day 235 post BMT.
Growth protocol Human OCI-Ly7 cell lines were cultured in IMDM supplemented with 15% FBS, 1% L-Glutamine and 1% penicillin-streptomycin.
Extracted molecule total RNA
Extraction protocol Total RNAs were extracted from sorted mouse cell suspensions using TRIzol LS Reagent (Invitrogen) or from human OCI-Ly7 cells using Rneasy mini plus kit (Qiagen) following the manufacturer’s instructions.
Libraries were made using the Illumina TruSeq stranded mRNA kit.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
 
Description D235_KHET_R4
ck_bcl2_gcb_featurecounts.merged.counts.tsv
Data processing nfcore RNA version 2.0
Assembly: mm10 and hg38
Supplementary files format and content: Combined gene count table
 
Submission date Feb 03, 2023
Last update date Feb 19, 2024
Contact name Christopher Russell Chin
E-mail(s) chc2077@med.cornell.edu
Phone 3393640514
Organization name Weill Cornell
Lab Melnick Lab
Street address 413 E 69th Street, Belfer Building, BB-1462
City New York City
State/province NY
ZIP/Postal code 10021
Country USA
 
Platform ID GPL24247
Series (2)
GSE224465 Cooperative super-enhancer inactivation caused by heterozygous loss of CREBBP and KMT2D skews B-cell fate decisions and accelerates development of T-cell depleted lymphomas [RNA-Seq]
GSE224513 Cooperative super-enhancer inactivation caused by heterozygous loss of CREBBP and KMT2D skews B-cell fate decisions and accelerates development of T-cell depleted lymphomas.
Relations
BioSample SAMN33054174
SRA SRX19275170

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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