|
Status |
Public on Feb 19, 2024 |
Title |
OCI-Ly7_WT_rep1 |
Sample type |
SRA |
|
|
Source name |
GCB-DLBCL
|
Organism |
Homo sapiens |
Characteristics |
cell line: GCB-DLBCL genotype: WT
|
Treatment protocol |
Mice (WT, C, K, and CK) were immunized with SRBC for 10 days before collecting CBs and CCs. Mice (BCL2, BCL2+C, BCL2+K, BCL+CK) were immunized with SRBC once every 3 weeks (starting at 56 days post-BMT) to induce continuous GC formation and lymphomagenesis, GC B cells were sorted at day 235 post BMT.
|
Growth protocol |
Human OCI-Ly7 cell lines were cultured in IMDM supplemented with 15% FBS, 1% L-Glutamine and 1% penicillin-streptomycin.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNAs were extracted from sorted mouse cell suspensions using TRIzol LS Reagent (Invitrogen) or from human OCI-Ly7 cells using Rneasy mini plus kit (Qiagen) following the manufacturer’s instructions. Libraries were made using the Illumina TruSeq stranded mRNA kit.
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Description |
WT_R1 ly7_featurecounts.merged.counts.tsv
|
Data processing |
nfcore RNA version 2.0 Assembly: mm10 and hg38 Supplementary files format and content: Combined gene count table
|
|
|
Submission date |
Feb 03, 2023 |
Last update date |
Feb 19, 2024 |
Contact name |
Christopher Russell Chin |
E-mail(s) |
chc2077@med.cornell.edu
|
Phone |
3393640514
|
Organization name |
Weill Cornell
|
Lab |
Melnick Lab
|
Street address |
413 E 69th Street, Belfer Building, BB-1462
|
City |
New York City |
State/province |
NY |
ZIP/Postal code |
10021 |
Country |
USA |
|
|
Platform ID |
GPL24676 |
Series (2) |
GSE224465 |
Cooperative super-enhancer inactivation caused by heterozygous loss of CREBBP and KMT2D skews B-cell fate decisions and accelerates development of T-cell depleted lymphomas [RNA-Seq] |
GSE224513 |
Cooperative super-enhancer inactivation caused by heterozygous loss of CREBBP and KMT2D skews B-cell fate decisions and accelerates development of T-cell depleted lymphomas. |
|
Relations |
BioSample |
SAMN33054193 |
SRA |
SRX19275149 |