|
Status |
Public on Jul 05, 2024 |
Title |
Alveolar macrophages from control mouse (sample #5), LPS |
Sample type |
SRA |
|
|
Source name |
Lung
|
Organism |
Mus musculus |
Characteristics |
tissue: Lung cell type: Primary alveolar macrophages genotype: Sftpc-CreERt2(+/-) treatment: Intra-tracheal LPS
|
Treatment protocol |
LPS (2 mg/kg; Sigma L2637) was given through intra-tracheal route to the murine lungs to induce acute lung injury.
|
Growth protocol |
NA
|
Extracted molecule |
total RNA |
Extraction protocol |
Cell as input and lysis accroding to manufacturer's instructions of SMART-Seq Stranded Kit (Takara) Libraries were prepared using the SMART-Seq Stranded Kit (Takara)
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Description |
N11020-20 read_count_Macrophage.txt
|
Data processing |
Trimming adaptor sequences and removing reads of low quality were performed by cutadapt (v2.4). Quantified reads were aligned to the mouse genome (GRCm38) by STAR (v2.7.2a) and then gene-level read counts were generated based on the annotations of Gencode (vM25) Assembly: GRCm38 Supplementary files format and content: tab-delimited text files include read count values for each sample
|
|
|
Submission date |
Feb 02, 2023 |
Last update date |
Jul 05, 2024 |
Contact name |
Kuei-Pin Chung |
Organization name |
National Taiwan University Hospital
|
Department |
Laboratory Medicine
|
Street address |
No 7, Chung-Shan South Rd
|
City |
Taipei |
ZIP/Postal code |
100 |
Country |
Taiwan |
|
|
Platform ID |
GPL24247 |
Series (1) |
GSE224354 |
Alveolar epithelial cells mitigate neutrophilic inflammation in acute lung injury through regulating mitochondrial fatty acid oxidation |
|
Relations |
BioSample |
SAMN33021190 |
SRA |
SRX19261144 |