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Sample GSM7020392 Query DataSets for GSM7020392
Status Public on Jul 05, 2024
Title Alveolar macrophages from control mouse (sample #5), LPS
Sample type SRA
 
Source name Lung
Organism Mus musculus
Characteristics tissue: Lung
cell type: Primary alveolar macrophages
genotype: Sftpc-CreERt2(+/-)
treatment: Intra-tracheal LPS
Treatment protocol LPS (2 mg/kg; Sigma L2637) was given through intra-tracheal route to the murine lungs to induce acute lung injury.
Growth protocol NA
Extracted molecule total RNA
Extraction protocol Cell as input and lysis accroding to manufacturer's instructions of SMART-Seq Stranded Kit (Takara)
Libraries were prepared using the SMART-Seq Stranded Kit (Takara)
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
 
Description N11020-20
read_count_Macrophage.txt
Data processing Trimming adaptor sequences and removing reads of low quality were performed by cutadapt (v2.4).
Quantified reads were aligned to the mouse genome (GRCm38) by STAR (v2.7.2a) and then gene-level read counts were generated based on the annotations of Gencode (vM25)
Assembly: GRCm38
Supplementary files format and content: tab-delimited text files include read count values for each sample
 
Submission date Feb 02, 2023
Last update date Jul 05, 2024
Contact name Kuei-Pin Chung
Organization name National Taiwan University Hospital
Department Laboratory Medicine
Street address No 7, Chung-Shan South Rd
City Taipei
ZIP/Postal code 100
Country Taiwan
 
Platform ID GPL24247
Series (1)
GSE224354 Alveolar epithelial cells mitigate neutrophilic inflammation in acute lung injury through regulating mitochondrial fatty acid oxidation
Relations
BioSample SAMN33021190
SRA SRX19261144

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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