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Status |
Public on Mar 31, 2013 |
Title |
WNV day 7 rep1 |
Sample type |
RNA |
|
|
Source name |
West Nile virus strain 2741
|
Organism |
Aedes aegypti |
Characteristics |
strain: Rockefeller disease state: West Nile strain 2741
|
Treatment protocol |
5-7 day post-eclosion Ae. aegypti mosquitoes were infected with WNV (strain 2741), YFV (Asibi strain) or DEN-2 (strain New Guinea C) via a 300 nL intra-thoracic inoculation of 5 log mosquito infectious dose 50% of virus
|
Growth protocol |
mosquitoes grown at 28 degrees C, 80% humidity
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA extracted using Rneasy kit, Qiagen
|
Label |
Cy3
|
Label protocol |
Labeling was performed by Yale University Keck Facilities (New Haven, CT) following the Nimblegen standard operating protocol. See www.nimblegen.com.
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|
|
Hybridization protocol |
Hybridization was performed by Yale University Keck Facilities (New Haven, CT) following the Nimblegen standard operating protocol. See www.nimblegen.com.
|
Scan protocol |
Scanning was performed by Yale University Keck Facilities (New Haven, CT) following the Nimblegen standard operating protocol. See www.nimblegen.com.
|
Description |
This sample is of RNA from day 7, Aedes aegypti mosquitoes infected with West Nile virus analyses on each experiment
|
Data processing |
Data normalization was quantile normalization. (reference: Bolstad B, et al. (Bioinformatics, 19:185-193, 2003)
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|
|
Submission date |
Apr 04, 2011 |
Last update date |
Mar 31, 2013 |
Contact name |
Tonya M Colpitts |
E-mail(s) |
tonya.colpitts@yale.edu
|
Phone |
203-737-5635
|
Fax |
203-785-6815
|
Organization name |
Yale University School of Medicine
|
Department |
Internal Medicine, Section of Infectious Diseases
|
Lab |
Erol Fikrig
|
Street address |
300 Cedar St, TAC S410
|
City |
New Haven |
State/province |
CT |
ZIP/Postal code |
06520 |
Country |
USA |
|
|
Platform ID |
GPL13335 |
Series (1) |
GSE28208 |
Analysis of the Aedes aegypti transcriptome during flavivirus infection |
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