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Sample GSM701590 Query DataSets for GSM701590
Status Public on Mar 31, 2013
Title WNV day 2 rep2
Sample type RNA
 
Source name West Nile virus strain 2741
Organism Aedes aegypti
Characteristics strain: Rockefeller
disease state: West Nile strain 2741
Treatment protocol 5-7 day post-eclosion Ae. aegypti mosquitoes were infected with WNV (strain 2741), YFV (Asibi strain) or DEN-2 (strain New Guinea C) via a 300 nL intra-thoracic inoculation of 5 log mosquito infectious dose 50% of virus
Growth protocol mosquitoes grown at 28 degrees C, 80% humidity
Extracted molecule total RNA
Extraction protocol RNA extracted using Rneasy kit, Qiagen
Label Cy3
Label protocol Labeling was performed by Yale University Keck Facilities (New Haven, CT) following the Nimblegen standard operating protocol. See www.nimblegen.com.
 
Hybridization protocol Hybridization was performed by Yale University Keck Facilities (New Haven, CT) following the Nimblegen standard operating protocol. See www.nimblegen.com.
Scan protocol Scanning was performed by Yale University Keck Facilities (New Haven, CT) following the Nimblegen standard operating protocol. See www.nimblegen.com.
Description This sample is of RNA from day 2, Aedes aegypti mosquitoes infected with West Nile virus analyses on each experiment
Data processing Data normalization was quantile normalization. (reference: Bolstad B, et al. (Bioinformatics, 19:185-193, 2003)
 
Submission date Apr 04, 2011
Last update date Mar 31, 2013
Contact name Tonya M Colpitts
E-mail(s) tonya.colpitts@yale.edu
Phone 203-737-5635
Fax 203-785-6815
Organization name Yale University School of Medicine
Department Internal Medicine, Section of Infectious Diseases
Lab Erol Fikrig
Street address 300 Cedar St, TAC S410
City New Haven
State/province CT
ZIP/Postal code 06520
Country USA
 
Platform ID GPL13335
Series (1)
GSE28208 Analysis of the Aedes aegypti transcriptome during flavivirus infection

Data table header descriptions
ID_REF
VALUE quantile normalized

Data table
ID_REF VALUE
AAEL000001-RA 58684.1
AAEL000002-RA 43146.5
AAEL000003-RA 50948.5
AAEL000004-RA 59348.3
AAEL000005-RA 15529.8
AAEL000005-RB 43707.8
AAEL000006-RA 49381.9
AAEL000007-RA 688.742
AAEL000008-RA 32468.6
AAEL000009-RA 5148.49
AAEL000010-RA 60750.8
AAEL000011-RA 613.919
AAEL000012-RA 321.469
AAEL000013-RA 18552.4
AAEL000014-RA 21515.6
AAEL000015-RA 36429
AAEL000016-RA 48397.9
AAEL000017-RA 3327.87
AAEL000018-RA 751.675
AAEL000019-RA 24861.3

Total number of rows: 15959

Table truncated, full table size 340 Kbytes.




Supplementary file Size Download File type/resource
GSM701590_263335A03_3-4-09_532.pair.gz 1.2 Mb (ftp)(http) PAIR
Processed data included within Sample table
Processed data are available on Series record

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