|
| Status |
Public on Mar 31, 2013 |
| Title |
WNV day 1 rep3 |
| Sample type |
RNA |
| |
|
| Source name |
West Nile virus strain 2741
|
| Organism |
Aedes aegypti |
| Characteristics |
strain: Rockefeller
disease state: West Nile strain 2741
|
| Treatment protocol |
5-7 day post-eclosion Ae. aegypti mosquitoes were infected with WNV (strain 2741), YFV (Asibi strain) or DEN-2 (strain New Guinea C) via a 300 nL intra-thoracic inoculation of 5 log mosquito infectious dose 50% of virus
|
| Growth protocol |
mosquitoes grown at 28 degrees C, 80% humidity
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extracted using Rneasy kit, Qiagen
|
| Label |
Cy3
|
| Label protocol |
Labeling was performed by Yale University Keck Facilities (New Haven, CT) following the Nimblegen standard operating protocol. See www.nimblegen.com.
|
| |
|
| Hybridization protocol |
Hybridization was performed by Yale University Keck Facilities (New Haven, CT) following the Nimblegen standard operating protocol. See www.nimblegen.com.
|
| Scan protocol |
Scanning was performed by Yale University Keck Facilities (New Haven, CT) following the Nimblegen standard operating protocol. See www.nimblegen.com.
|
| Description |
This sample is of RNA from day 1, Aedes aegypti mosquitoes infected with West Nile virus analyses on each experiment
|
| Data processing |
Data normalization was quantile normalization. (reference: Bolstad B, et al. (Bioinformatics, 19:185-193, 2003)
|
| |
|
| Submission date |
Apr 04, 2011 |
| Last update date |
Mar 31, 2013 |
| Contact name |
Tonya M Colpitts |
| E-mail(s) |
tonya.colpitts@yale.edu
|
| Phone |
203-737-5635
|
| Fax |
203-785-6815
|
| Organization name |
Yale University School of Medicine
|
| Department |
Internal Medicine, Section of Infectious Diseases
|
| Lab |
Erol Fikrig
|
| Street address |
300 Cedar St, TAC S410
|
| City |
New Haven |
| State/province |
CT |
| ZIP/Postal code |
06520 |
| Country |
USA |
| |
|
| Platform ID |
GPL13335 |
| Series (1) |
| GSE28208 |
Analysis of the Aedes aegypti transcriptome during flavivirus infection |
|
