|
| Status |
Public on Mar 13, 2023 |
| Title |
CXCR1-CD14+ monocyte |
| Sample type |
SRA |
| |
|
| Source name |
PB leukocytes
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: PB leukocytes
cell subtype: CXCR1-CD14+ monocyte
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from sorted cells was extracted with the TRIzol LS reagent.
RNA-seq libraries were prepared using the Nextera XT Kit (Illumina) according to the manufacturer's protocol.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
Sequencing reads were aligned to a human reference genome in the Bowtie software (version 1.1.2).
Duplicate reads were removed using Picard tools.
Expression was quantitated using Cufflinks software.
Assembly: hg38/GRCh38
Supplementary files format and content: EXCEL file includes FPKM values for all samples.
|
| |
|
| Submission date |
Jan 31, 2023 |
| Last update date |
Jan 03, 2024 |
| Contact name |
Kenichi Asano |
| E-mail(s) |
asanok@toyaku.ac.jp
|
| Organization name |
Tokyo University of Pharmacy and Life Sciences
|
| Department |
Life Sciences
|
| Lab |
Immune Regulation
|
| Street address |
1432-1 Horinouchi
|
| City |
Hachioji |
| State/province |
Tokyo |
| ZIP/Postal code |
1920392 |
| Country |
Japan |
| |
|
| Platform ID |
GPL18573 |
| Series (2) |
| GSE224101 |
Human CXCR1+CD14+ monocytes are characterized by neutrophil-associated gene expression [RNA-seq human] |
| GSE224702 |
mouse RNA-seq, human RNA-seq, ATAC-seq, and scRNA-seq analyses data |
|
| Relations |
| Reanalyzed by |
GSE252426 |
| BioSample |
SAMN32971694 |
| SRA |
SRX19226826 |
