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Sample GSM700063 Query DataSets for GSM700063
Status Public on Mar 31, 2011
Title Th2
Sample type RNA
Source name primary CD4 T cells from spleen
Organism Mus musculus
Characteristics strain: C57BL/6
tissue: spleen
cell type: CD4 T cell
culture conditions: Th2
Treatment protocol Th1 and Th2 culture cells were collected and resuspendet in the Trizol solution (GibcoBRL).
Growth protocol Splenic CD4 T cells were prepared using a magnetic cell sorter (AutoMACS; Miltenyi Biotec) yielding a purity of >98%. Where indicated, cells from C57BL/6 mice were stimulated with immobilized anti-TCR mAb (H57−597; 3 mg/ml) and anti-CD28 mAb under Th1- or Th2-culture conditions for 2days in vitro. Th1 conditions; 25 U/ml IL-2, 10 U/ml IL-12, and antiミIL-4 mAb. Th2 conditions; 25 U/ml IL-2, 100 U/ml IL-4. pMX-IRES-NGFR retrovirus were added to the Th1 and Th2 cell differentiation cultures on day 2. After culturing for 3 more days, the cells were harvested.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Total RNA was extracted using the IVT Labeling kit from Affymetrix
Hybridization protocol aRNA was hybridized to GeneChip Mouse Genome 430 2.0 Arrays from Affymetrix according the manufacturers protocol.
Scan protocol GeneChips were scanned using the GeneChip Scanner 3000.
Description Gene expression data from wild type CD4+ T cells cultured for 5 days in Th2 condition
Data processing Expression values were determined using GeneChip Operating Software (GCOS) v1.2 software.
Submission date Mar 31, 2011
Last update date Aug 04, 2011
Contact name Atsushi Onodera
Organization name Chiba University
Department Immunology
Street address Chuo-ku Inohana 1-8-1
City Chiba
ZIP/Postal code 260-8670
Country Japan
Platform ID GPL1261
Series (1)
GSE28292 Genome-wide analysis reveals unique regulation of transcription of Th2-specific genes by GATA3

Data table header descriptions
VALUE MAS5.0 signal intensity

Data table
1415670_at 817.8
1415671_at 513.5
1415672_at 1362
1415673_at 660.5
1415674_a_at 552.6
1415675_at 204.5
1415676_a_at 1392.5
1415677_at 107.9
1415678_at 337.9
1415679_at 1085.5
1415680_at 595.3
1415681_at 620.7
1415682_at 202.7
1415683_at 1445.3
1415684_at 158.8
1415685_at 340
1415686_at 295.2
1415687_a_at 591
1415688_at 564.1
1415689_s_at 133.1

Total number of rows: 45101

Table truncated, full table size 711 Kbytes.

Supplementary file Size Download File type/resource
GSM700063.CEL.gz 3.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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