GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM700062 Query DataSets for GSM700062
Status Public on Mar 31, 2011
Title Th1
Sample type RNA
Source name primary CD4 T cells from spleen
Organism Mus musculus
Characteristics strain: C57BL/6
tissue: spleen
cell type: CD4 T cell
culture conditions: Th1
Treatment protocol Th1 and Th2 culture cells were collected and resuspendet in the Trizol solution (GibcoBRL).
Growth protocol Splenic CD4 T cells were prepared using a magnetic cell sorter (AutoMACS; Miltenyi Biotec) yielding a purity of >98%. Where indicated, cells from C57BL/6 mice were stimulated with immobilized anti-TCR mAb (H57−597; 3 mg/ml) and anti-CD28 mAb under Th1- or Th2-culture conditions for 2days in vitro. Th1 conditions; 25 U/ml IL-2, 10 U/ml IL-12, and antiミIL-4 mAb. Th2 conditions; 25 U/ml IL-2, 100 U/ml IL-4. pMX-IRES-NGFR retrovirus were added to the Th1 and Th2 cell differentiation cultures on day 2. After culturing for 3 more days, the cells were harvested.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Total RNA was extracted using the IVT Labeling kit from Affymetrix
Hybridization protocol aRNA was hybridized to GeneChip Mouse Genome 430 2.0 Arrays from Affymetrix according the manufacturers protocol.
Scan protocol GeneChips were scanned using the GeneChip Scanner 3000.
Description Gene expression data from wild type CD4+ T cells cultured for 5 days in Th1 condition
Data processing Expression values were determined using GeneChip Operating Software (GCOS) v1.2 software.
Submission date Mar 31, 2011
Last update date Aug 04, 2011
Contact name Atsushi Onodera
Organization name Chiba University
Department Immunology
Street address Chuo-ku Inohana 1-8-1
City Chiba
ZIP/Postal code 260-8670
Country Japan
Platform ID GPL1261
Series (1)
GSE28292 Genome-wide analysis reveals unique regulation of transcription of Th2-specific genes by GATA3

Data table header descriptions
VALUE MAS5.0 signal intensity

Data table
1415670_at 642.8
1415671_at 538
1415672_at 1278.4
1415673_at 359
1415674_a_at 446.5
1415675_at 193.1
1415676_a_at 1022.1
1415677_at 161.4
1415678_at 308.3
1415679_at 987.3
1415680_at 478.3
1415681_at 571
1415682_at 181
1415683_at 1129.3
1415684_at 116.6
1415685_at 243.6
1415686_at 184.2
1415687_a_at 712.3
1415688_at 494.2
1415689_s_at 97.7

Total number of rows: 45101

Table truncated, full table size 708 Kbytes.

Supplementary file Size Download File type/resource
GSM700062.CEL.gz 3.4 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap