|
| Status |
Public on Mar 31, 2011 |
| Title |
Th1 |
| Sample type |
RNA |
| |
|
| Source name |
primary CD4 T cells from spleen
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
tissue: spleen
cell type: CD4 T cell
culture conditions: Th1
|
| Treatment protocol |
Th1 and Th2 culture cells were collected and resuspendet in the Trizol solution (GibcoBRL).
|
| Growth protocol |
Splenic CD4 T cells were prepared using a magnetic cell sorter (AutoMACS; Miltenyi Biotec) yielding a purity of >98%. Where indicated, cells from C57BL/6 mice were stimulated with immobilized anti-TCR mAb (H57−597; 3 mg/ml) and anti-CD28 mAb under Th1- or Th2-culture conditions for 2days in vitro. Th1 conditions; 25 U/ml IL-2, 10 U/ml IL-12, and antiﾐIL-4 mAb. Th2 conditions; 25 U/ml IL-2, 100 U/ml IL-4. pMX-IRES-NGFR retrovirus were added to the Th1 and Th2 cell differentiation cultures on day 2. After culturing for 3 more days, the cells were harvested.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Total RNA was extracted using the IVT Labeling kit from Affymetrix
|
| |
|
| Hybridization protocol |
aRNA was hybridized to GeneChip Mouse Genome 430 2.0 Arrays from Affymetrix according the manufacturers protocol.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000.
|
| Description |
Gene expression data from wild type CD4+ T cells cultured for 5 days in Th1 condition
|
| Data processing |
Expression values were determined using GeneChip Operating Software (GCOS) v1.2 software.
|
| |
|
| Submission date |
Mar 31, 2011 |
| Last update date |
Aug 04, 2011 |
| Contact name |
Atsushi Onodera |
| Organization name |
Chiba University
|
| Department |
Immunology
|
| Street address |
Chuo-ku Inohana 1-8-1
|
| City |
Chiba |
| ZIP/Postal code |
260-8670 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE28292 |
Genome-wide analysis reveals unique regulation of transcription of Th2-specific genes by GATA3 |
|
