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Sample GSM699727 Query DataSets for GSM699727
Status Public on May 24, 2012
Title HP1-gamma_HCT116-WT_rep1
Sample type SRA
Source name human colorectal carcinoma cell line HCT116
Organism Homo sapiens
Characteristics genotype/variation: Wild type
cell line: HCT116
stimulation: none
chip antibody: HP1 gamma
antibody vendor: Millipore
antibody catalog number: 05-690
antibody lot numbers: 1501782 and 17908001
alignment program: ELAND
Treatment protocol Cells were treated with 10 ng/ml TNF-alpha for 30-60 mins.
Growth protocol HCT116 cells were grown in McCoys media, supplemented with 10% FBS and 1% pen-strep.
Extracted molecule genomic DNA
Extraction protocol See
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina Genome Analyzer II
Data processing ChIP-Seq reads were mapped by the Eland to hg18, keeping only uniquely mapping genes and discarding all but one of clonal reads. Bedgraph files were generated using bed files as the input files. Only chromosome positions with at least 5 monoclonal reads are retained.
Submission date Mar 30, 2011
Last update date May 15, 2019
Contact name Bing Ren
Organization name University of California, San Diego
Street address 9500 Gilman Drive
City La Jolla
State/province CA
ZIP/Postal code 92093
Country USA
Platform ID GPL9115
Series (1)
GSE28115 CBX3 Regulates Efficient RNA Processing Genome Wide
SRA SRX055346
BioSample SAMN00255088

Supplementary file Size Download File type/resource
GSM699727.bed.gz 288.7 Mb (ftp)(http) BED
GSM699727.bedgraph.gz 93.0 Mb (ftp)(http) BEDGRAPH
SRA Run SelectorHelp
Processed data provided as supplementary file
Raw data are available in SRA

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