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Sample GSM694169 Query DataSets for GSM694169
Status Public on Oct 01, 2011
Title Control 9
Sample type RNA
Source name IB3 cells untransfected
Organism Homo sapiens
Characteristics cell line: IB3-1
cell type: Bronchial epithelial cell line
transfection: Control
Treatment protocol CFTR and mutated forms were subcloned into the mammalian expression vector, pCDNA 3.1 and transfected into cells using Lipofectamine 2000 (Invitrogen)
Growth protocol IB3-1 cells (CF genotype _F508/W1282X, bronchial epithelial derivation), were grown in LHC-8 Basal Medium, 95%; fetal bovine serum, 5%.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol Following fragmentation, 15 ug of cRNA were hybridized for 16 hr on GeneChip the U133 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the AffyMetrix GeneChip Scanner
Description Gene expression data untreated IB3 cells.
Data processing Microarray scans were processed in GCOS 1.2 to determine expression levels using Affymetrix default analysis settings and global scaling as normalization method.The trimmed mean target intensity of each array was set to 100.
Submission date Mar 21, 2011
Last update date Oct 01, 2011
Contact name Jerry M. Wright
Phone 410-614-5010
Organization name Johns Hopkins Medical Institutions
Department Physiology
Street address 725 N. Wolfe St
City Baltimore
State/province MD
ZIP/Postal code 21205
Country USA
Platform ID GPL96
Series (1)
GSE28072 Expression data from transfected IB3 cells transfected with CFTR mutants
Reanalysis of GSM9514

Data table header descriptions
VALUE MAS 5.0 signal intensity, trimmed mean target intensity of each array was set to 100

Data table
1007_s_at 193.01994 P
1053_at 230.47049 P
117_at 11.33707 A
121_at 358.5614 P
1255_g_at 14.783618 A
1294_at 71.40368 P
1316_at 31.191755 P
1320_at 21.247633 A
1405_i_at 27.783932 P
1431_at 2.822325 A
1438_at 31.876482 A
1487_at 207.36111 P
1494_f_at 61.85595 A
1598_g_at 288.90912 P
160020_at 199.33015 P
1729_at 71.35949 M
1773_at 55.885326 A
177_at 46.338306 P
179_at 258.1285 P
1861_at 103.76808 P

Total number of rows: 22283

Table truncated, full table size 488 Kbytes.

Supplementary file Size Download File type/resource
GSM694169_GSM9514.CEL.gz 3.4 Mb (ftp)(http) CEL
Processed data included within Sample table
Processed data are available on Series record

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