|
| Status |
Public on May 24, 2024 |
| Title |
HDMB03_Ctrl_2 |
| Sample type |
SRA |
| |
|
| Source name |
T Milde et al., J Neurooncol 2012
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: Human HDMB03 group 3 medulloblastoma cell line
treatment: scramble control
|
| Growth protocol |
Tumorspheres were grown in StemPro NSC Serum-Free Medium on ultra-low attachment tissue culture plates
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from all samples using the Norgen RNA extraction kit (Norgen Biotek),
Library constructed with NEB Ultra II directional RNA library prep kit with poly-A selection module
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
Aligned with STAR v2.5.2b
Assembly: Hg19
Supplementary files format and content: count matrix (txt. File)
|
| |
|
| Submission date |
Jan 12, 2023 |
| Last update date |
May 24, 2024 |
| Contact name |
Liam D Hendrikse |
| Organization name |
University Health Network
|
| Department |
Princess Margaret Cancer Centre
|
| Street address |
610 University Ave
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5G 2C4 |
| Country |
Canada |
| |
|
| Platform ID |
GPL18573 |
| Series (1) |
| GSE222699 |
The OTX2-regulated alternative splicing landscape controls differentiation and reconstitutes the developmental origins of Group 3 medulloblastoma |
|
| Relations |
| BioSample |
SAMN32691261 |
| SRA |
SRX19013819 |
