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Sample GSM692822 Query DataSets for GSM692822
Status Public on Sep 14, 2011
Title S3: corneas dissected 3 days after sham operated (lens left in) at stage 50
Sample type RNA
 
Source name corneas dissected 3 days after sham operated (lens left in) at stage 50
Organism Xenopus laevis
Characteristics developmental stage: stage 50, Nieuwkoop and Faber
genotype: wildtype
tissue: cornea
Growth protocol tadpoles were cultured at 20 degrees in 0.1 x MMR salts overnight and then in 25 degrees in water until harvested at day 3 after surgery
Extracted molecule total RNA
Extraction protocol RNA later, Ambion RNAqueous micro kit used to extract RNA and treat with DNAse I as protocol.
Label biotin
Label protocol Nugen WT-Ovation pico kit
 
Hybridization protocol WS2v4_450
Scan protocol 7G Plus GeneChip Scanner 3000
Description XenLensRegS3
Data processing RMA normalised using GenePattern software
 
Submission date Mar 17, 2011
Last update date Sep 14, 2011
Contact name Caroline Beck
E-mail(s) caroline.beck@otago.ac.nz
Organization name University of Otago
Department Zoology
Street address 340 Great King Street
City Dunedin
ZIP/Postal code 9054
Country New Zealand
 
Platform ID GPL1318
Series (1)
GSE28014 Identification of genes associated with lens regeneration from the cornea in Xenopus laevis tadpoles

Data table header descriptions
ID_REF
VALUE RMA signal

Data table
ID_REF VALUE
AFFX-BioB-3_at 148.3456
AFFX-BioB-5_at 82.17014
AFFX-BioB-M_at 108.99738
AFFX-BioC-3_at 233.68285
AFFX-BioC-5_at 156.583
AFFX-BioDn-3_at 1466.2821
AFFX-BioDn-5_at 353.9106
AFFX-CreX-3_at 3324.9836
AFFX-CreX-5_at 2282.8206
AFFX-DapX-3_at 2.1586792
AFFX-DapX-5_at 1.0311713
AFFX-DapX-M_at 0.821991
AFFX-LysX-3_at 0.89504725
AFFX-LysX-5_at 1.030406
AFFX-LysX-M_at 1.0854487
AFFX-PheX-3_at 1.7888992
AFFX-PheX-5_at 0.5708423
AFFX-PheX-M_at 1.155485
AFFX-r2-Bs-dap-3_at 0.965656
AFFX-r2-Bs-dap-5_at 0.92803144

Total number of rows: 15611

Table truncated, full table size 403 Kbytes.




Supplementary file Size Download File type/resource
GSM692822_XenLensRegS3.CEL.gz 1.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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