|
| Status |
Public on Sep 14, 2011 |
| Title |
R1: corneas dissected 3 days after removal of lens at stage 50 |
| Sample type |
RNA |
| |
|
| Source name |
corneas dissected 3 days after removal of lens at stage 50
|
| Organism |
Xenopus laevis |
| Characteristics |
developmental stage: stage 50, Nieuwkoop and Faber
genotype: wildtype
tissue: cornea
|
| Growth protocol |
tadpoles were cultured at 20 degrees in 0.1 x MMR salts overnight and then in 25 degrees in water until harvested at day 3 after surgery
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA later, Ambion RNAqueous micro kit used to extract RNA and treat with DNAse I as protocol.
|
| Label |
biotin
|
| Label protocol |
Nugen WT-Ovation pico kit
|
| |
|
| Hybridization protocol |
WS2v4_450
|
| Scan protocol |
7G Plus GeneChip Scanner 3000
|
| Description |
XenLensRegR1
|
| Data processing |
RMA normalised using GenePattern software
|
| |
|
| Submission date |
Mar 17, 2011 |
| Last update date |
Sep 14, 2011 |
| Contact name |
Caroline Beck |
| E-mail(s) |
caroline.beck@otago.ac.nz
|
| Organization name |
University of Otago
|
| Department |
Zoology
|
| Street address |
340 Great King Street
|
| City |
Dunedin |
| ZIP/Postal code |
9054 |
| Country |
New Zealand |
| |
|
| Platform ID |
GPL1318 |
| Series (1) |
| GSE28014 |
Identification of genes associated with lens regeneration from the cornea in Xenopus laevis tadpoles |
|
