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Sample GSM692816 Query DataSets for GSM692816
Status Public on Sep 14, 2011
Title L2: whole lens excised stage 50
Sample type RNA
 
Source name whole lens excised stage 50
Organism Xenopus laevis
Characteristics developmental stage: stage 50, Nieuwkoop and Faber
genotype: wildtype
tissue: whole lens
Growth protocol tadpoles were cultured at 20 degrees in 0.1 x MMR salts overnight and then in 25 degrees in water until harvested at day 3 after surgery
Extracted molecule total RNA
Extraction protocol RNA later, Ambion RNAqueous micro kit used to extract RNA and treat with DNAse I as protocol.
Label biotin
Label protocol Nugen WT-Ovation pico kit
 
Hybridization protocol WS2v4_450
Scan protocol 7G Plus GeneChip Scanner 3000
Description XenLensRegL2
Data processing RMA normalised using GenePattern software
 
Submission date Mar 17, 2011
Last update date Sep 14, 2011
Contact name Caroline Beck
E-mail(s) caroline.beck@otago.ac.nz
Organization name University of Otago
Department Zoology
Street address 340 Great King Street
City Dunedin
ZIP/Postal code 9054
Country New Zealand
 
Platform ID GPL1318
Series (1)
GSE28014 Identification of genes associated with lens regeneration from the cornea in Xenopus laevis tadpoles

Data table header descriptions
ID_REF
VALUE RMA signal

Data table
ID_REF VALUE
AFFX-BioB-3_at 212.80342
AFFX-BioB-5_at 155.36568
AFFX-BioB-M_at 184.20058
AFFX-BioC-3_at 319.33585
AFFX-BioC-5_at 254.31752
AFFX-BioDn-3_at 1397.0063
AFFX-BioDn-5_at 398.80093
AFFX-CreX-3_at 2560.7976
AFFX-CreX-5_at 2083.9104
AFFX-DapX-3_at 2.5586932
AFFX-DapX-5_at 1.2174256
AFFX-DapX-M_at 1.1566225
AFFX-LysX-3_at 1.8757991
AFFX-LysX-5_at 1.142653
AFFX-LysX-M_at 1.3684219
AFFX-PheX-3_at 4.332399
AFFX-PheX-5_at 6.040411
AFFX-PheX-M_at 1.8248643
AFFX-r2-Bs-dap-3_at 1.0528963
AFFX-r2-Bs-dap-5_at 1.6156803

Total number of rows: 15611

Table truncated, full table size 402 Kbytes.




Supplementary file Size Download File type/resource
GSM692816_XenLensRegL2.CEL.gz 1.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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