We carried out expression profiling experiments in which gene expression in logarithmically growing cultures of HDAC mutant cells was compared to that in wt controls. Wt, hos2D, clr6-1, clr3D and sir2D cells were grown to midlogarithmic phase (5*10power6 cells/ml) in rich medium, and RNA was extracted and subjected to cDNA expression profiling analysis using the established protocols (Xue et al, 2004).
