|
Status |
Public on Jan 23, 2023 |
Title |
Flnc global KO sample 3 |
Sample type |
SRA |
|
|
Source name |
whole heart
|
Organism |
Mus musculus |
Characteristics |
tissue: whole heart developmental stage: E9.5 genotype: Flnc global knockout strain: C57BL/6
|
Extracted molecule |
polyA RNA |
Extraction protocol |
E9.5 embryonic hearts were homogenized in TRIzol (Invitrogen) and total RNA was isolated according to the manufacturer’s instructions. cDNA libraries were prepared using an Illumina TruSeq stranded mRNA kit according to manufacturer’s instructions.
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Data processing |
Sequencing reads were mapped to GENCODE mouse transcripts reference (release M22, GRCm38.p6) and transcription levels were quantified using salmon. Differential expression analysis was carried out using DEseq2 (version: 1.22.2). Benjamini-Hochberg correction for multiple testing was applied to correct p-value of each gene as false discovery rate (FDR). FDR < 0.05 was used as a threshold for differentially-expressed genes (DEGs). Assembly: mm10 Supplementary files format and content: Tab-delimited text files include TPM and reads count for each sample
|
|
|
Submission date |
Jan 10, 2023 |
Last update date |
Jan 23, 2023 |
Contact name |
Ju Chen |
E-mail(s) |
juchen@health.ucsd.edu
|
Organization name |
University of California San Diego
|
Street address |
9500 Gilman Drive 0613
|
City |
La Jolla |
State/province |
California |
ZIP/Postal code |
92122 |
Country |
USA |
|
|
Platform ID |
GPL24247 |
Series (1) |
GSE222542 |
Filamin C is Essential for Mammalian Myocardial Integrity |
|
Relations |
BioSample |
SAMN32660420 |
SRA |
SRX18989470 |