|
| Status |
Public on May 31, 2011 |
| Title |
adipose_tissue_DM (16167) |
| Sample type |
RNA |
| |
|
| Source name |
subcutaneous adipose tissue
|
| Organism |
Homo sapiens |
| Characteristics |
clinical status: DM
bmi: 28.8
age: 64
fasting insulin: 88
fasting glucose: 11.7
hba1c: 7
vo2max ffm: 32
|
| Treatment protocol |
Adipose tissue biopsies were obtained from the subcutaneous abdominal region (from the area below the umbilicus in a 4-cm range on either side) using the percutaneous needle biopsy technique with suction. Biopsies were quickly dissected free from visible blood and connective tissue and frozen in liquid nitrogen. To avoid interference of circadian rhythms and post-prandial responses, biopsies were obtained between 8 am and 10 am following an overnight fast.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Frozen tissue was homogenised in Trizol (Invitrogen) using a motor-driven homogenizer (Polytron, Kinematica, Newark, NY, USA). RNA was extracted according to manufacturer’s protocol.
|
| Label |
biotin
|
| Label protocol |
As per Affymetrix directions
|
| |
|
| Hybridization protocol |
As per Affymetrix directions
|
| Scan protocol |
As per Affymetrix directions
|
| Data processing |
All array data were normalized using the Microarray Suite version 5.0 (MAS 5.0) algorithm to a global scaling intensity of 100. Data were filtered using present/absent calls. Probes called absent by MAS 5.0 across all arrays were discarded. Further data processing were performed by RMA.
|
| |
|
| Submission date |
Mar 14, 2011 |
| Last update date |
May 31, 2011 |
| Contact name |
Iain Gallagher |
| E-mail(s) |
iaingallagher@gmail.com
|
| Phone |
00 44 1786 46 6024
|
| Organization name |
University of Stirling
|
| Department |
Faculty of Health Sciences and Sport
|
| Street address |
Room 4B133 Cottrell Building, University of Stirling, Airthrey Road
|
| City |
Stirling |
| ZIP/Postal code |
FK9 4LA |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE27949 |
Gene-chip studies of adipogenesis-regulated microRNAs in mouse primary adipocytes and human obesity (Affymetrix) |
| GSE27951 |
Gene-chip studies of adipogenesis-regulated microRNAs in mouse primary adipocytes and human obesity |
|
