|
| Status |
Public on Dec 20, 2005 |
| Title |
02_sp_a_2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Tissue Type: Spleen
|
| Organism |
Mus musculus |
| Characteristics |
Age: 8 weeks
Sex: male
Strain/Cell-line: C57Bl/6J
Developmental stage: Adult
|
| Extracted molecule |
total RNA |
| Label |
Cy5
|
| Label protocol |
direct labelling of cDNA
|
| |
|
| Channel 2 |
| Source name |
Common Reference: 17.5dpc total embryo
|
| Organism |
Mus musculus |
| Characteristics |
17.5dpc total embryo
|
| Extracted molecule |
total RNA |
| Label |
Cy3
|
| Label protocol |
direct labelling of cDNA
|
| |
|
| |
| Scan protocol |
Image Analysis Software: DigitalGENOME (Molecularware Inc.)
|
| Description |
Experiment Type: Expression profile of each tissue
Dye Swap: red/green
Organization: RIKEN Genome Science Centre (GSC) Yokohama Japan
|
| Data processing |
Lowess Normalisation (Genespring7.2). A Lowess curve was fit to the log-intensity versus log-ratio plot. 20.0% of the data was used to calculate the Lowess fit at each point. This curve was used to adjust the control value for each measurement. If the control channel was lower than 10 then 10 was used instead. Each gene was divided by the median of its measurements in all samples. If the median of the raw values was below 10 then each measurement for that gene was divided by 10 if the numerator was above 10 otherwise the measurement was thrown out.
|
| |
|
| Submission date |
Aug 09, 2005 |
| Last update date |
Dec 20, 2005 |
| Contact name |
Darrin F Taylor |
| E-mail(s) |
d.taylor@imb.uq.edu.au
|
| Phone |
+61 7 3346 2607
|
| Organization name |
Institute for Molecular Bioscience, Uni of Qld
|
| Department |
Genomics and Bioinformatics
|
| Lab |
Expression Genomics Lab
|
| Street address |
Research Rd
|
| City |
St Lucia |
| State/province |
Qld |
| ZIP/Postal code |
4072 |
| Country |
Australia |
| |
|
| Platform ID |
GPL2733 |
| Series (1) |
| GSE3098 |
Systematic expression profiling of the mouse transcriptome using RIKEN cDNA microarrays |
|
