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Sample GSM686630 Query DataSets for GSM686630
Status Public on Mar 15, 2011
Title NIH3T3_Rapamycin_18hr_rep3
Sample type RNA
 
Source name NIH3T3, Rapamycin, 18hr, replicate 3
Organism Mus musculus
Characteristics cell line: NIH3T3
treatment: 50 nM Rapamycin
Treatment protocol NIH3T3 cells were treated with DMSO, 50 nM rapamycin, or 2 mM PP242 for 18 hours
Growth protocol NIH3T3 cells were cultured in DMEM supplemented with 10% BCS and penicillin/streptomycin.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using QIAshredder and RNeasy Mini kits (Qiagen). RNA was quantified by Nanodrop and integrity was assessed on an Agilent 2100 Bioanalyzer.
Label Cy3
Label protocol RNA was amplified using the whole transcriptome amplification kits (Sigma) following the manufacturer’s protocol, and subsequent Cy3-CTP labeling was performed using one-color labeling kits (NimbleGen). The size distribution and quantity of the amplified product was assessed by Nanodrop and Bioanalyzer.
 
Hybridization protocol Equal amounts of Cy3 labeled target were hybridized to Agilent mouse whole genome 4x44K Ink-jet arrays. Hybridizations were performed for 14 hrs, according to the manufacturers protocol.
Scan protocol Arrays were scanned using the Agilent microarray scanner.
Description Gene expression in NIH3T3 cells after 18hr treatment with 50 nM Rapamycin
Data processing Raw signal intensities were extracted with Feature Extraction v10.1 software. The dataset was normalized using the quantile normalization method. No background subtraction was performed, and the median feature pixel intensity was used as the raw signal before normalization.
 
Submission date Mar 07, 2011
Last update date Mar 15, 2011
Contact name Beatrice Wang
E-mail(s) beatrice.wang@ucsf.edu
Organization name Univeristy of California, San Francisco
Street address 600 16th Street MC 2280
City San Francisco
State/province CA
ZIP/Postal code 94158
Country USA
 
Platform ID GPL7202
Series (1)
GSE27784 Transcriptional profiling of ATP-competitive mTOR inhibitors reveals mTORC1 and mTORC2 specific regulatory networks

Data table header descriptions
ID_REF
VALUE quantile normalized signal intensity

Data table
ID_REF VALUE
A_52_P616356 6.078595603
A_52_P580582 6.941985612
A_52_P403405 5.757112167
A_52_P819156 7.375039431
A_51_P331831 7.122827995
A_51_P430630 7.75627832
A_52_P502357 6.758223215
A_52_P299964 7.370324145
A_51_P356389 6.108524457
A_52_P684402 8.48112669
A_51_P414208 6.093637638
A_51_P280918 8.325867581
A_52_P613688 6.136991112
A_52_P258194 7.471675214
A_52_P229271 6.563831821
A_52_P214630 7.887968183
A_52_P579519 9.648657176
A_52_P979997 5.836050355
A_52_P453864 6.712527
A_52_P655842 7.180323598

Total number of rows: 41174

Table truncated, full table size 998 Kbytes.




Supplementary file Size Download File type/resource
GSM686630_251486829328_S01_GE1_105_Jan09_1_4.txt.gz 2.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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