|
| Status |
Public on Dec 23, 2022 |
| Title |
IG6_01302017 |
| Sample type |
SRA |
| |
|
| Source name |
Plasma
|
| Organism |
Homo sapiens |
| Characteristics |
donor: EXR-IGHIR1DONOR2SL-DO
disease state: Healthy
tissue: Plasma
concentration lysed rbc: 0.05%
anatomical location: Entire cardiovascular system
rna source: total cell-free biofluid RNA
rna isolation kit: miRcury biofluids (Exiqon)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA Isolation kit was miRcury biofluids (Exiqon).
Library construction kit was NEBNext small RNA library prep (NEB). Samples were not multiplexed. Type of Starting Material was total RNA.
|
| |
|
| Library strategy |
ncRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
EXR-IGHIR1IG601302017-BS
|
| Data processing |
All FASTQ files were processed uniformly through the extra-cellular RNA processing toolkit (exceRpt), with an adapter sequence of [] and random barcode length [] (on []). Information about exceRpt is available at https://github.gersteinlab.org/exceRpt/.
Assembly: hg19
Supplementary files format and content: *.tar: Each processed data file is a core results archive generated by exceRpt. The core results archive contains read counts for alignments to the different transcriptome libraries used by exceRpt (miRNA / piRNA / tRNA / GENCODE annotations / circRNA), taxonomy trees for exogenous rRNA and genomic reads aligned by exceRpt, and a variety of other files generated by exceRpt.
|
| |
|
| Submission date |
Dec 19, 2022 |
| Last update date |
Dec 24, 2022 |
| Contact name |
Ionita Calin Ghiran |
| E-mail(s) |
ionitaghiran@gmail.com
|
| Phone |
617-735-4114
|
| Organization name |
Beth Israel Deaconess Medical Center
|
| Department |
Medicine
|
| Street address |
3 Blackfan Circle
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL18573 |
| Series (1) |
| GSE221312 |
Impact of Hb contamination in obtaining reference profiles of exRNAs in healthy individuals |
|
| Relations |
| BioSample |
SAMN32310722 |
| SRA |
SRX18771317 |
