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| Status |
Public on Jan 01, 2024 |
| Title |
Mitral valve, inflamed, 8 weeks |
| Sample type |
SRA |
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| Source name |
Mitral valve
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| Organism |
Mus musculus |
| Characteristics |
tissue: Mitral valve
genotype: K/B.g7
age: 8 weeks
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| Extracted molecule |
total RNA |
| Extraction protocol |
Mitral valves were isolated from B.g7 and K/B.g7 hearts and digested using Collagenase 2 and DNAse. Following digestion, cells were washed and filtered, then red blood cells lysed using AKC buffer. Cells were stained with a viability dye and live, single cells were sorted using fluorescence-activated cell sorting (FACS).
Library was performed according to the manufacter’s instructions (single cell 3’ protocol, v2, 10x Genomics).
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic single cell |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
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| Data processing |
The demultiplexing, barcoded processing, gene counting and aggregation were made using the Cell Ranger software v3.1.0.
Assembly: mm10-3.0.0
Supplementary files format and content: Tab-separated values files and matrix files
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| Submission date |
Dec 16, 2022 |
| Last update date |
Jan 01, 2024 |
| Contact name |
Bryce A Binstadt |
| E-mail(s) |
binstadt@umn.edu
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| Organization name |
University of Minnesota
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| Department |
Pediatrics
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| Street address |
2-200 WMBB Bench 8
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| City |
Minneapolis |
| State/province |
Minnesota |
| ZIP/Postal code |
55455 |
| Country |
USA |
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| Platform ID |
GPL24247 |
| Series (1) |
| GSE221197 |
Cellular composition of murine mitral valves during autoimmune valvular carditis |
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| Relations |
| BioSample |
SAMN32279134 |
| SRA |
SRX18752927 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM6855673_KBxN_8wk_barcodes.tsv.gz |
50.2 Kb |
(ftp)(http) |
TSV |
| GSM6855673_KBxN_8wk_features.tsv.gz |
272.8 Kb |
(ftp)(http) |
TSV |
| GSM6855673_KBxN_8wk_matrix.mtx.gz |
60.3 Mb |
(ftp)(http) |
MTX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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