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Sample GSM67661

Query DataSets for GSM67661

Status

Public on Aug 11, 2005

Title

IGR clr3-myc binding map(#14)

Sample type

genomic

Channel 1

Source name

CHIP IP clr3-myc

Organism

Schizosaccharomyces pombe

Characteristics

clr3-myc sample

Extracted molecule

genomic DNA

Label

cy3

Channel 2

Source name

CHIP Input

Organism

Schizosaccharomyces pombe

Characteristics

CHIP input clr3-myc

Extracted molecule

genomic DNA

Label

cy5

Description

For HDAC binding (Clr3-myc and Sir2- myc), ChIP was performed according to Kurdistani et al (2002), also with the modification to the protocol of leaving out the cold PBS wash. We used 1.5–3.0 mg 9E10 monoclonal antibody against Myc(Sigma) for each IP.

Data processing

cy3/cy5

Submission date

Aug 08, 2005

Last update date

Oct 28, 2005

Contact name

Karl Ekwall

E-mail(s)

karl.ekwall@ki.se

Phone

+46 8 6089133

Organization name

Karolinska Inst

Street address

Alfred Nobels Alle 7

City

Stockholm

ZIP/Postal code

S-141 89

Country

Sweden

Platform ID

GPL2553

Series (1)

GSE2879

Genomewide analysis of nucleosome density histone acetylation and HDAC function in fission yeast

Data table header descriptions
ID_REF
VALUE	log2 of PRE_VALUE
PRE_VALUE

Data table
ID_REF	VALUE	PRE_VALUE
1	1.8918	3.711
2	3.3491	10.19
3	3.1473	8.86
4	2.6355	6.214
5	1.9119	3.763
6	1.6758	3.195
7	1.1947	2.289
8	2.3360	5.049
9	0.5666	1.481
10	1.3791	2.601
11	-0.0469	0.968
12	2.9596	7.779
13	0.8852	1.847
14	1.8327	3.562
15	2.3153	4.977
16	3.3291	10.05
17	2.3763	5.192
18	1.7446	3.351
19	3.6100	12.21
20	1.6402	3.117

Total number of rows: 10286

Table truncated, full table size 184 Kbytes.

Supplementary data files not provided