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Sample GSM675133 Query DataSets for GSM675133
Status Public on Dec 31, 2011
Title 1C5_tun
Sample type RNA
Source name U87 glioma cells
Organism Homo sapiens
Characteristics cell line: U87-MG
plasmid: pCDNA3.1_IRE1DN
treatment: upon tunicamycin treatment
Treatment protocol cells were washed with ice cold PBS
Growth protocol U87 control and IRE1DN cells subjected to severe hypoxia (0.1%), glucose deprivation, glutamine deprivation and tunicamycin treatment (5ug/ml) for 16h
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions. RNA was purified on Quiagen columns
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133A 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol standard Affy protocol
Description Gene expression data from U87_IRE1DNcells upon tunicamycin treatment
Data processing GCOS 1.2 software (Affymetrix).
Submission date Feb 14, 2011
Last update date Dec 31, 2011
Contact name eric chevet
Organization name inserm
Department U1053
Street address 146 rue Leo Saignat
City Bordeaux
ZIP/Postal code 33076
Country France
Platform ID GPL571
Series (1)
GSE27306 IRE1-dependent transcriptome remodelling upon ER stress in human glioma cells

Data table header descriptions
VALUE signal intensity

Data table
AFFX-BioB-5_at 58.9
AFFX-BioB-M_at 66.7
AFFX-BioB-3_at 48.6
AFFX-BioC-5_at 199.5
AFFX-BioC-3_at 275.6
AFFX-BioDn-5_at 379.7
AFFX-BioDn-3_at 738
AFFX-CreX-5_at 2024.3
AFFX-CreX-3_at 2461.2
AFFX-DapX-5_at 36.2
AFFX-DapX-M_at 63.7
AFFX-DapX-3_at 69.6
AFFX-LysX-5_at 3.3
AFFX-LysX-M_at 7.9
AFFX-LysX-3_at 14.8
AFFX-PheX-5_at 5.1
AFFX-PheX-M_at 4.1
AFFX-PheX-3_at 7.5
AFFX-ThrX-5_at 8
AFFX-ThrX-M_at 9.2

Total number of rows: 22277

Table truncated, full table size 339 Kbytes.

Supplementary file Size Download File type/resource
GSM675133.CEL.gz 2.1 Mb (ftp)(http) CEL
Processed data included within Sample table
Processed data are available on Series record

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