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Sample GSM675132 Query DataSets for GSM675132
Status Public on Dec 31, 2011
Title 1C5GLUT
Sample type RNA
 
Source name U87 glioma cells
Organism Homo sapiens
Characteristics cell line: U87-MG
plasmid: pCDNA3.1_IRE1DN
treatment: under glutamine deprivation
Treatment protocol cells were washed with ice cold PBS
Growth protocol U87 control and IRE1DN cells subjected to severe hypoxia (0.1%), glucose deprivation, glutamine deprivation and tunicamycin treatment (5ug/ml) for 16h
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions. RNA was purified on Quiagen columns
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133A 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol standard Affy protocol
Description Gene expression data from U87_IRE1DN cells under glutamine deprivation
Data processing GCOS 1.2 software (Affymetrix).
 
Submission date Feb 14, 2011
Last update date Dec 31, 2011
Contact name eric chevet
E-mail(s) eric.chevet@u-bordeaux2.fr
Organization name inserm
Department U1053
Street address 146 rue Leo Saignat
City Bordeaux
ZIP/Postal code 33076
Country France
 
Platform ID GPL571
Series (1)
GSE27306 IRE1-dependent transcriptome remodelling upon ER stress in human glioma cells

Data table header descriptions
ID_REF
VALUE signal intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 46.9
AFFX-BioB-M_at 64.3
AFFX-BioB-3_at 38.8
AFFX-BioC-5_at 142.2
AFFX-BioC-3_at 186.4
AFFX-BioDn-5_at 282.4
AFFX-BioDn-3_at 549.2
AFFX-CreX-5_at 1602.6
AFFX-CreX-3_at 1750.2
AFFX-DapX-5_at 63
AFFX-DapX-M_at 121.8
AFFX-DapX-3_at 122.5
AFFX-LysX-5_at 9.8
AFFX-LysX-M_at 14.6
AFFX-LysX-3_at 29.7
AFFX-PheX-5_at 12.2
AFFX-PheX-M_at 15.6
AFFX-PheX-3_at 16.6
AFFX-ThrX-5_at 18
AFFX-ThrX-M_at 28.3

Total number of rows: 22277

Table truncated, full table size 340 Kbytes.




Supplementary file Size Download File type/resource
GSM675132.CEL.gz 2.0 Mb (ftp)(http) CEL
Processed data included within Sample table
Processed data are available on Series record

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