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Sample GSM675131 Query DataSets for GSM675131
Status Public on Dec 31, 2011
Title 1C5HG
Sample type RNA
Source name U87 glioma cells
Organism Homo sapiens
Characteristics cell line: U87-MG
plasmid: pCDNA3.1_IRE1DN
treatment: under glucose deprivation
Treatment protocol cells were washed with ice cold PBS
Growth protocol U87 control and IRE1DN cells subjected to severe hypoxia (0.1%), glucose deprivation, glutamine deprivation and tunicamycin treatment (5ug/ml) for 16h
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions. RNA was purified on Quiagen columns
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133A 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol standard Affy protocol
Description Gene expression data from U87_IRE1DN cells under glucose deprivation
Data processing GCOS 1.2 software (Affymetrix).
Submission date Feb 14, 2011
Last update date Dec 31, 2011
Contact name eric chevet
Organization name inserm
Department U1053
Street address 146 rue Leo Saignat
City Bordeaux
ZIP/Postal code 33076
Country France
Platform ID GPL571
Series (1)
GSE27306 IRE1-dependent transcriptome remodelling upon ER stress in human glioma cells

Data table header descriptions
VALUE signal intensity

Data table
AFFX-BioB-5_at 48.1
AFFX-BioB-M_at 61.8
AFFX-BioB-3_at 37.1
AFFX-BioC-5_at 144.6
AFFX-BioC-3_at 187.4
AFFX-BioDn-5_at 279.8
AFFX-BioDn-3_at 569.3
AFFX-CreX-5_at 1581.5
AFFX-CreX-3_at 1838.6
AFFX-DapX-5_at 62.3
AFFX-DapX-M_at 126.8
AFFX-DapX-3_at 136.9
AFFX-LysX-5_at 8.3
AFFX-LysX-M_at 10.7
AFFX-LysX-3_at 34
AFFX-PheX-5_at 17.8
AFFX-PheX-M_at 15.3
AFFX-PheX-3_at 14.4
AFFX-ThrX-5_at 20.2
AFFX-ThrX-M_at 28.7

Total number of rows: 22277

Table truncated, full table size 339 Kbytes.

Supplementary file Size Download File type/resource
GSM675131.CEL.gz 2.0 Mb (ftp)(http) CEL
Processed data included within Sample table
Processed data are available on Series record

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